| 1. | By lac z screen , 19 positive colonies were found
进一步做lacz鉴定， 19个菌落为阳性，其中7个菌落为强阳性。 |
| 2. | The recombinant vector was digested with tthllll and the lacz gene from e . coli was inseted in this site , the generated plasmid is designated as pltk - uni
然后定向亚克隆swha基因于多克隆位点，获得重组转移载体pltk - ha 。 |
| 3. | ( 4 ) in order to investigate the influence of the carbon source on the expression of cryld - lacz , cryld - lacz was introduced into different strains and cultured in liquid medium containing different carbon sources
检测了在苏云金芽胞杆菌不同菌株中，不同碳源对cry1d - lacz融合基因表达的影响。 |
| 4. | The percent of larvae expressing lacz gradually increased for various time points after inoculation , but the percent of larvae expressing lacz reduced after 20 hours post inoculation , we thought the infection spots transformed
、从卜又’荞!乡;三、卜率却有所降低，对此，我们分析其原因可能是由于病毒的感染部位发生了转移。 |
| 5. | The transient expression of lacz driven by crtw 306bp s ' - flanking sequence and crtz 302bp s ' - flanking sequence shows that these two sequences habour transcription regulatory sequences
以lacz为报告基因的瞬间表达实验结果表明，长度分别为306bp和302bp的crtw和crtz5 '上游侧翼序列具有很强的启动转录活性，提示两段序列包含了启动子的结构。 |
| 6. | ( 2 ) influence of the promoter and upstream region on expression of cry id was tested . firstly , the pcr products of cry id promoter and its upstream were obtained followed by the construction of shuttle plasmid containing cryld - lacz
尽管cry1dmrna比cry1abmrna的半衰期更长，但cry1d和cry1ab转录时间和转录量的差异是导致其表达量差异的重要原因。 |
| 7. | Sodium succinate as carbon source could lead to earlier sporulation and earlier expression of cryld - lacz , when sodium acetate acted as carbon source , cryid - lacz could keep high , stable expression in different hosts
琥珀酸钠作碳源时导致芽胞形成提前， cry1d - lacz融合基因表达也随之提前。而以乙酸钠作碳源时，在不同亚种的菌株中都能高水平、持续稳定地表达。 |
| 8. | When cotransforming pgbd - nifa and ad fusion genomic library into saccharomyces cerevisiae pj69 - 4a , 109 candidates interacting with nifa had been selected by testing for the expression of the his3 , ade2 and lacz reporter genes
诱饵质粒和文库质粒共转化酿酒酵母( saccharomycescerevisiae ) pj69 - 4a ，通过检测报告基因his3 、 ade2及lacz的表达进行筛选，初筛得到109个阳性酵母菌落。 |
| 9. | Analysis of activity of p - galactosidase showed that cryld - lacz expressed differently in the same host because of different carbon sources and differenly in different hosts utlizing same carbon sources , suggesting that metabolism pathway of carbon source in hd - 133 - 5 was special
首次报道了cry1d - lacz在同一菌株中的表达因碳源不同而异，在碳源相同的条件下不同菌株的表达也不相同，而衍生菌株hd - 133 - 5的代谢途径非常特殊。 |
| 10. | Fixed with gluteraldehyde , stained with ph 7 . 4 phosphate buffered x - gal dye solution , observed by reverse microscope , the results are as follow : the percent of larvae expressing lacz in midgut showed that fb - 28 improved the sensitivity of larvae to virus
病毒感染后的8h ， 12h ， 16h ， 20h提取中肠组织，按常规石蜡包埋切片进行洗涤、透明、浸蜡、包埋、切片、粘片等操作程序，制成连续切片，在倒置显微镜下逐一进行观察计数。 |
