| 1. | Construction of recombinant lactococcus lactis expressing porcine transmissible gastroenteritis spike glycoprotein and analysis of immunogenicity
基因的重组乳酸乳球菌的构建及免疫原性分析 |
| 2. | Sds - page analysis revealed that lactococcus lactis nz9800 harbouring pmg36e / nisa restored little ability of nisin production
以lactococcuslactisnz9800为受体菌，研究了表达载体的表达情况。 |
| 3. | In this paper , functional kefir yogurt starter was produced by lactococcus and lactobacillus isolated from kefir grains
摘要本文利用开菲尔粒中分离的乳球菌和乳杆菌采用混合发酵法研制开菲尔酸奶复合发酵剂。 |
| 4. | Using a mouse model , the uk team has also found that a modified bacterium lactococcus lactis can be used to vaccinate against harmful infections
利用小鼠模型，该英国研究小组已经发现了一种基因修饰细菌（乳酸乳球菌）可用于对有害传染病的免疫接种。 |
| 5. | 4 . the recombinant plasmid pmg36e / nisa was introduced into lactococcus lactis nz9800 . sds - page analysis revealed that lactococcus lactis nz9800 harbouring pmg36e / nisa restored no ability of nisin production
抽提ecolijm109中的重组质粒，转化l . lactisnz9800 ，在含红霉素的gm17平板上筛选转化子。 |
| 6. | This has resulted in the availability of genetically modified l . lactis with prospects for application in food industry . in this studay , genomic dna extracted from lactococcus lactis nizo r5 was used directly as the template for pcr in this paper
乳酸乳球菌是一类长期应用于食品发酵业的有益微生物，以乳酸菌为受体的表达系统具有安全、表达产物便于分离，使用方便等优点。 |
| 7. | A dna fragment was amplified from lactococcus lactis nizo r5 genome by means of pcr and cloned into pmg36e plasmid . the recombinant plasmid was transformd into e . colijm 109 and identified by restriction endonucleases analyzing . the recombinant plasmid was introduced into lactococcus lactis nz9800
本实验提取乳酸乳球菌nizor5的基因组dna为模板，用pcr方法得到乳链菌肽前体基因( nisa ) ，克隆到乳酸乳球菌表达载体pmg36e ，构建了表达载体pmg36e nisa 。 |
| 8. | Compared with lactococcus , lactobacillus is the better choice of oral vaccine for some properties , including their immunogenicities , resistance to the circumstance of the alimentary tract and remaining in long period in gastro - intestinal tract , however , lactococcus is more effective in selecting antigen to induce immunological response , while the positive result of lactobacillus is limited
与乳酸乳球菌相比，乳酸杆菌特有的一些性质使其成为口服疫苗更好的选择，这些特性包括它们的免疫原性，对上消化道内情况的耐受以及能在胃肠道中长时间留存，然而乳球菌在选择抗原诱导免疫反应方面更加有效，但是乳杆菌的阳性结果十分有限。 |
| 9. | A approximately 460bp dna fragment was amplified by pcr from lactococcus lactis nizo r5 genome . the primers used in this study comprised the following nucleotide sequences : 5 " - cgcgaattcgatataggtttattgagt - 3 " and 5 " - atgaagcttatccatgtcagaactaa - 3 " . conditions used for the pcr consisted of 30 cycles of 94 ? for0 . 5min , 45 ? for imin and 72 ? for 1 . 5min , plus one additional cycle of 72 for 10min
根据已发表的乳链菌肽前体基因的dna序列，设计两条引物并引入酶切位点。以lactococcuslactisr5基因组dna为模板， pcr反应条件： 94变性30s ， 45退火60s ， 72延伸90s ，反应进行30个循环。成功扩出一条约460bp的dna片段。 |
