| 1. | Cloning and sequence analysis of - carotene hydroxylase gene fro
胡萝卜素羟化酶基因的克隆和序列分析 |
| 2. | Tyrosine hydroxylase , th mrna
酪氨酸羟化酶 |
| 3. | Exercise also decreased striatal da transporter and tyrosine hydroxylase immunoreactivity in mptp - lesioned mice
运动也可以减少纹状体多巴胺转运体，还能抑制酪氨酸羟化酶的免疫反应性。 |
| 4. | Flavonoid - 3 ' , 5 ' - hydroxylase ( f3 ' , 5 ' h ) corresponding to the genetic loci hfl and hf2 , a member of the cytochrome p450 family , is the key enzyme in the anthocyanin biosynthesis , which is generally required for blue or purple flowers
本研究以紫蓝色矮牵牛基因组dna为模板，利用pcr技术获得了两个扩增产物，分别将其克隆至pgem - teasyvector上，进行了全序列分析。 |
| 5. | Studies have shown that the marginal division is associated with memory and learning . the distribution of dopamine and tryosine hydroxylase immunoreactivity in the rat striatum marginal division was here investigated by using immunohistochemistry abc method
本实验主要应用免疫组织化学方法研究多巴胺在边缘区的分布情况，从递质水平探讨多巴胺对纹状体边缘区学习与记忆的调控机制。 |
| 6. | The mechanism of memory and learning in the mrd performed by dopamine and its receptors was discussed . methods and results the immunohistochemistry method was used to investigate the distribution of dopamine and tryosine hydroxylase immunoreactivity in the marginal division of rat striatum using da and th polyclone antibody
但在纹状体边缘区中的da和th免疫阳性纤维较多，呈密集的带状分布，且有蝌蚪状终末，沿背腹方向走行，未见胞体。 |
| 7. | A homolog of ga sbeta - hydroxylase gene expression pattern was examined . hybridization signals of this gene could be detected at day 5 and however , they appear again after day 10 when explants cultured with hormones . it has the similar pattern when the explants cultured without hormones or uncultured
风信子ga羟基化酶类似基回在外植体表达，在含有外源撒素条件下培养的前5天受到激素的抑制而几乎不表达，第10天开始在花分生组织中表达。 |
| 8. | Based on cdna sequences of ( 3 - carotene ketolase ( cr / ff ) and p - carotene hydroxylase ( c / - fz ) , a 1 . 6 kb crtw genomic sequence with six introns , and a 3kb crtz genomic sequence with five introns were clone , respectively . all the exon - intron junctions conform closely to gu - ag consensus splicing rule
本论文工作成功地克隆两个关键酶基因的基因组序列，发现crtw和crtz分别包括6个和5个内含子序列，所有这11个内含子的剪切位点都符合gu - ag规律。 |
| 9. | Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium . osmoregulation positions in the brain , reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ) , oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos , glial fibrillary acidic protein ( gfap ) , tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining , immuno - electronic microscope , wga - hrp retrogradely tracing and cell culture methods . results : ( 1 ) fos positive neurons within the mvz , parabrachial nuclei , locus ceruleus , pvn , son , subfomical organ increased markedly
方法：通过给予大鼠饮用3氯化钠或提高培养基渗透压浓度的方法复制高渗刺激模型，主要采用抗fos 、胶质原纤维酸性蛋白( gfap )和酪氨酸羟化酶( th ) (或加压素? vp )免疫组织化学多重染色、免疫电镜、 wga - hrp束路追踪结合免疫组织化学多重染色、细胞培养等实验方法，系统观察了中枢参与渗透压反射的调控部位、下丘脑视上核( son )神经元? ast超微结构的变化、延髓内脏带( mvz )和son及下丘脑室旁核( pvn )之间往返投射通路和神经元的性质及其与ast的关系、培养神经元和ast内钙波的变化。 |
| 10. | Phenylketonuria ( pku ) is an inherited metabolic disease that results in mental retardation and other neurological problems when treatment is not started within the first few weeks of life . the disease arises from the deficiency of a single enzyme , phenylalanine hydroxylase , which converts the essential amino acid , phenylalanine , to another amino acid , tyrosine . failure of the conversion to take place results in a buildup of phenylalanine in the body that then damages the central nervous system
苯丙酮尿症（ pku ）是一种智力发育不全的先天性疾病，患者由于肝赃内苯丙氨酸羟化酶缺乏，苯丙氨酸不能正常代谢为酪氨酸，从而导致苯丙氨酸在肌体组织内积累，引起脑损伤和累进性精神障碍，临床表现为智力低下,头发颜色转黄，尿有异臭味，重者似鼠臭。 |
