Successful treatment of recurrent histiocytic necrotic lymphnoditis 复发性组织细胞性坏死性淋巴结炎治验
2.
Section two the evaluation of biocompatibility of the acellular dermal matrix by the method of cell culture . the new born rat ' s epdermic cells were cultured with the acellular dermal matrix together as experiment group , while the epdermic cell were cultured simply as control . 24 hours later , under the invert microscope , the epidermic cells anchored well and transparent flat cells were observed in both groups . 7 days later , both cultured cells were taked out and fixed in 95 % ethanol , stained with hematoxylin and were observed under light microscope . many cleaved cells were observed in both groups . during cell culture , no pathogenic microganism was observed . so we considered the acellular dermal matrix was aseptic and had good biocompatibility . section three subdermal implantation of the acellular dermal matrix . 24 rats were used in the experiments . a piece of acellular dermal matrix ( 1 . 5 x 1 . 5cm2 ) was implanted beneath the dorsum skin flaps of each rat , 1 week , 2 weeks , 3 weeks and 4 weeks after implantation , 6 pieces of acellular dermal matrix were harvested and the size of implanted acellular dermal matrix were measured , the sections were used for he staining and observed under light microscope . the result were as folio wing : 1 - 2 weeks after implantation , the acellular dermal matrix began to adhere to the tissue around and turned red gradually ; 3 - 4weeks after implantation , the acellular dermal matrix adhered closely to the tissue around and could be recognized easily , 1 - 3 weeks after implantation , the size of implanted acellular dermal matrix had no statistical difference ( p > 0 . 05 ) . 4 weeks after implantation implanted acellular dermal matrix contracted ( p < 0 . 05 ) . under light microscope , l - 2weeks after implantation , the fibroblast cells infiltrated the acellular dermal matrix and a small amount endothelial cells of vessel and lympho - histiocytic cells infiltrated the acellular dermal matrix . 3 - 4 weeks after implantation , infiltrating blood vessels were evident . so we think that the acellular dermal matrix had low immunological reactions and could induce the infiltration of fibroblast macrophage cell and the endothelial cells of vessel 结果如下：皮下包埋卜周者，无细胞真皮基质渐与周围组织粘附，颜色由苍白转红；皮下包埋3周者，无细胞真皮基质与周围组织紧密枯附，盾晰叶辩；术后卜周，包埋的基质面积变化较包埋前无统计学差异o川0引，术后4周包埋的无细胞真皮基质面积较包埋前缩小j刃刀5 ） 。光镜下术后卜周，宿主的淋巳组织细胞、成纤维细胞浸入生长，釉附在胶原纤维上，少量血管内皮细胞浸入基质；术后34周，无细胞真皮基质内较多的血管形成，故可认为无细胞真皮基质免疫原性低，能诱导宿主的成纤维细胞、巨噬细胞浸入生长，为一种新型的真皮替代物。第四部分无细胞真皮基质与自体断层皮片复合移棺的研究， sd大鼠10只，在其背部卜方造成全厚皮肤缺损的创面
