| 1. | It was very impossible that those mutations of gx epidemic strains of csfv can cause the incompleted protection of hclv vaccine
基因的克隆与序列分2729 、 734和738位氨基酸均发生了变异。 |
| 2. | Computer - base phylogenetic tree among the two strains of gx and other 16 strains mentioned above were obtained by analyzing nucleotide sequences of e2 gene
这些变异极有可能使广西流行野毒逃避hclv的免疫保护。 gxnn 、 gxlz的e 。基因与已知序列的16株国内外毒株的e 。 |
| 3. | The 100 % immuno - protection against hcv strain f114 , v - jn and v - yc was observed in the experimental piglets that had been vaccinated with hclv strain vaccine by the program above
攻毒试验再一次证实仔猪按此程序2次免疫，于150日龄攻毒时试验组均未发病，保护率100 。 |
| 4. | And another nine strains and strains hclv , shimen of two major reference strains of china belonged to group i . in addition , strain p97 was the special individual
G皿z 、 gxnn 、与属1群的中国主要参考毒株hclv 、 shimen在遗传关系上较远，与italy株和paderborn株比较接近。 |
| 5. | Hc ( hog cholera ) , the virulent infectious disease caused by hcv ( hog cholera virus ) , had been controlled effectively by hclv strain vaccine in the country
猪瘟是由猪瘟病毒引起的猪的一种病毒性传染病。猪瘟兔化弱毒疫苗有效地控制了猪瘟在我国的急性发生和大流行。 |
| 6. | The homologies of genomic nucleotide sequence and deduced amino acid sequence of csfv gxwz02 with that of shimen , hclv were low , indicating that gxwz02 was not only genetically different from shimen and hclv but antigenically also
Gxwz02与shimen 、 hclv的全基因组序列及推定的氨基酸序列同源性有明显变异，表明gxwz02与shimen 、 hclv在遗传性和抗原性上存在较大差异。 |
| 7. | The result shows that the high degree of homology of e2 sequences and 426 residues amino acid sequences existed in the strain hclv , hcv - jn , hcv - yc , fc and the epitopes between the four strains are not variable obviously by analysising the variation of some main amino acid residues substitutions of e2 major antigenic domains , confirming initially immunization with hcv vaccine strain can resist challenging of field strains
结果发现， hclv与fc间核苷酸及氨基酸序列的同源性分别为98 . 3 、 99 . 2 ；两个野毒株与hclv和fc株之间的同源性也分别在98以上，与alfort株的同源性最低，分别在83 、 89左右。据此，可推测hclv 、猪瘟野毒株和c株之间的e2基因序列及氨基酸序列无明显差异。 |
| 8. | 1280bp cdna sequence of two hcv e2 sequences sequenced and 426 residues amino acid sequences including e2 glycoprotein were deduced . then the e2 gene nucleotide sequence and amino acid residues sequence were compared respectively with hclv , strain fc , strain shimen , strain alfort of france , strain brescia of italy and so on
随后对其进行了核苷酸序列测定和氨基酸序列的推导，并与测得的hclv 、国外测得的c株( fc )及我国的shimen株、法国的alfort株等的相应序列进行了同源性比较。 |
| 9. | A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv
以猪瘟兔化弱毒犊牛睾丸细胞毒( hclv )中提取的细胞总rna为模板，以rt - pcr技术，扩增出了完整e2基因的cdna片段。经电泳、酶切分析，证实了所扩增片段为e2基因特异性片段。 |
| 10. | The immuno - antibody against hcv of healthy piglets , which had been vaccinated with hclv strain vaccine in different period and dose , were detected by ppa - elisa . the results showed that the immuno - protection were acquired by the immunized piglets which had been vaccinated 2 doses at o day or 4 doses at 25 * day firstly and 4 doses at 60 * day secondly
选择健康仔猪于不同日龄、不同免疫剂量接种hclv细胞苗，再分别于不同时期检测抗体，结果表明， 0日龄2头份或25日龄4头份首免， 60日龄加强免疫4头份可使仔猪在整个育肥期得到有效的免疫保护。 |
