The embryonic shield appeared in 15h , and the fertilized egg entered the middle gastrula stage 15小时出现胚盾， 15小时55分，胚体下包一半。
2.
The fertil ized egg entered the early gastrula stage in 11h45min , the germ ring appeared , and the oil globules diffused in the plant hemisphere in this stage 11小时45分囊胚开始下包，胚环出现，油球出现重新扩散的现象。
3.
The main differences in ldh exist in five stages from middle - gastrula to muscle construction , which showed four more bands ( ldh9 " , ldh10 " , ldh 11 " and ldh 12 " ) in the isozyme pattern of embryo activated by heterogeneous sperm than that activated by homogenous sperm 而在est和me同工酶表达上，同源和异源精子激发的胚胎则几乎没有差异。对不同精子激发彭泽鲫雌核发育子代的生长效应研究，证实了异精效应的存在，发现海鲤精子是一种激发效果较好的外源精子。
4.
Recombination gene was cut - down and introduced into the nuclei of oocytes or the cytoplasm of goldfish at one - cell stage via microinjection . the results as follows : ( 1 ) fluorescence was observed from embryo under suitable uv light after microinjection 36 hours . the fluorescent ratio of gastrula embryo period was up to 25 % 采用显微注射法将这种重组基因转化1细胞期的金鱼受精卵，实验结果如下： ( 1 )显微注射后，根据胚胎发育分期，胚胎在显微注射后36小时开始能在紫外灯下观察到荧光，原肠期发荧光的胚胎比例为25 ，后期发育荧光率逐渐下降，肌肉效应期后又相对稳定。
5.
G - banding the gtg banding ( g - banding ) was carried out by the standard trypsin method with slight modification , which works well for protochordate because a good number of reproducible g - bands are consistently obtained from the embryonic cells of late blastulae and early gastrulae of amphioxus b . belcheri tsingtauense G带型用稍作修改的标准的胰酶显带技术，进行gtg带纹的显示，它们能较好地显示头索动物青岛文昌鱼的晚期囊胚和早期原肠胚的中期染色体的g带，并且重复性好。
