Study on fmoc solid - phase synthesis and activity of thymosin 1的固相合成及体外活性研究
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Determine amino acid contents with opa - fmoc pre - column derivatization rp - hplc 柱前衍生反相液相色谱法测定氨基酸含量
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In order to prove hwtx - i that can be used as one of natural scaffolds for protein engineering . we have synthesized two multiple mutants : hw - ma and rgd - ma and natural co - conotoxin mviia by using solid phase method with fmoc chemistry on peptide synthesizer and by hand respectively 为了证明hwtx -可以作为蛋白质工程研究的一种天然的骨架分子,本文运用fmoc化学,用手工和固相合成仪合成了两个hwtx -的多个位点的嵌合体: hw - ma及rgd - hw ,以及合成了天然- conotoxinmviia 。