Put this fusion gene under the control of the promoter of ie1 gene , and then an eucaryotic cell expression plasmid vector pgem / ie1 " - gfp - actin resulted 将该融合基因置于ie1基因启动子的控制之下，构建成了真核表达质粒pgem ie1 - gfp - actin 。
2.
Results after successfully construction of the eucaryotic expression vector , fertilized eggs were microinjected with linear expression vector and transferred into recipient mice , 34 mice were born and survival 经受精卵原核显微注射后移植到受体母鼠，第一批移植受体后产仔34只，经pcr检测阳性为3只。
3.
Actin is a high - conserved protein with a wb of 43kd , aniversally exists in eucaryotic cell . f - actin is polymerized by g - actin which participates the formation of the netlike protein system of the complex skeleton in the eucaryotic cell 微丝( f - actin )是由肌动蛋白单体( g - actin )聚合形成的，它参与了真核细胞中复杂的细胞骨架网状蛋白体系的形成。
4.
On the other hand , - 6 fatty acid desaturase injection assay will be performed to check whether a metabolic pathway is established . methords of research three plasmids vector with expression elements are used to establish a eucaryotic expression vector by restriction enzyme cutting and ligation . this vector is used to pronucleus microinjection 本实验以pegfp - n1质粒为骨架载体，用酶切连接的方法构建一个顺序含有- actin启动子、 fad2cdna 、 sv40polya加尾信号的真核表达载体，双切线性化后回收，使用回收的表达载体经原核显微注射生产转基因小鼠。
5.
Since the important roles of eo protein in the viral infection . immunity and virus - host interaction . the homology of 21 csfv strains was investigated by sequence analysis of eo genes in this study , which will provide some evidence for epidemiological study . in addition , the eo gene of hog cholera lapinized vaccine ( hclv ) strain was expressed in the prokaryotic and eucaryotic systems , and the recombinant proteins were preliminarily analyzed by immunological method 鉴于eo蛋白在病毒感染，诱导机体免疫及与宿主细胞相互关系中的作用，本研究克隆了2株猪瘟病毒eo基因并将其与其它毒株eo基因进行了序列分析，揭示了我国猪瘟病毒流行株之间的遗传演化关系，为猪瘟病毒的流行病学研究提供依据。
6.
Classical swine fever ( csf ) is one of major contagious diseases in pig breeding industry . in order to gain massive diagnostic antigen csfv , and large scale of csfv specific major protective antigen protein ( e2 envelope glycoprotein ) in eucaryotic cells , which are prepared into subunit vaccine for effective prevention against csf 猪瘟是危害养猪业的主要传染病，为了得到大量的猪瘟病毒特异性诊断抗原，并试图在真核细胞中大量表达猪瘟病毒特有的免疫保护性抗原，即e2囊膜糖蛋白，为进一步制备猪瘟亚单位疫苗有效预防本病奠定基础。
