They are the molecular markers of embryonic calli and embryogenetic stage correspondingly . besides , the content of 53kd increased in the embryogenetic stage , which implied that the protein 53kd might contribute to the development of embryoids 另外， 53kd蛋白组分的表达量在胚状体发育阶段明显增加，该种蛋白组分可能也对胚状体的发育起到了促进作用。
2.
Among them , the protein 36kd expressed in the period of c6 , embryonic calli , embryogenetic stage while not in nonembryonic calli and regenerated plants . it ' s a crucial protein in the determination of embryonic calli and the development of embryoids 其中36kd蛋白组分在胚性愈伤组织和胚状体中表达而在非胚性愈伤组织和再生植株中不表达，它是直接与愈伤组织胚性发生能力相关的特异性蛋白。
3.
One corresponded to the period of c6 in which there were some preparations for forming embryonic calli , the other corresponded to the embryogenetic stage . in the conversion of embryonic calli , the lowest threshold value of total protein content in embryonic calli was 11 . 5 + 0 . 5 ug / mg fw 它们分别对应于体细胞胚发生过程中胚性愈伤形成的准备期( c _ 6期)和胚状体发育期( e _ 1 e _ 5 )两个关键时期。
4.
Aralia elate ( miq . ) seem is a kind of uncultivated vegetable from the mountain changbai . the process of somatic embryogenesis includes morphogenesis stage of calli , conversion stage of embryonic calli , embryogenetic stage and regeneration stage of plant in aralia elate ( 本论文采用sds -聚丙烯酰胺凝胶电泳技术，对龙芽?木体细胞胚发生过程中愈伤组织形态建成、胚性愈伤转化、胚状体发育和植株再生四个阶段的可溶性蛋白的动态变化进行分析。
5.
In addition , a specific calli - related protein 43kd were found and its expression only occurred in the proliferation of calli . we also found a kind of nonembryonic protein 35kd that expressed in nonembryonic calli and regenerated plants and disappeared in embryonic calli and embryogenetic stage 此外，我们还发现，与愈伤组织增殖有关的43kd愈伤组织特异性蛋白；在胚性愈伤组织和胚状体发生阶段特异性缺失的35kd蛋白组分，它可能与胚状体发生能力的丧失有关。
6.
Surprisingly , heterogeneous sperm from other species of fish , besides stimulating and activating development of the egg , are able to contribute to the phenotype of gynogenetic offspring , that is allgynogenesis . the patterns of expressions in five isozymes ( est , mdh , me , sod and ldh ) were investigated in the embryogenetic stages of gynogenetic crucian carp ( carassius auratus of pengze ) activated by homogenous and heterogeneous sperms Mdh的差异有两点，在同源精子激发的胚胎中， mdh6 ’表达至心博期后消失，但在异源精子激发的胚胎中， mdh6 ’持续表达至孵出期；在孵出期，异源精子激发的胚胎比同源精子激发的胚胎多出两条带mdh4 ’和mdh5 ’ 。
