Recent studies have indicated the existence of dynein heavy chain and light chain in higher plants , but no data about dynein intermediate chain in higher plants have been reported 近来的研究表明在高等植物细胞中存在细胞质力蛋白的重链和轻链，但力蛋白中间链在高等植物细胞中还未见报道。
2.
These results suggest that the cytoplasmic dynein intermediate chain - like protein exists in lily pollen , and it distributes mainly on either cis - and trans - golgi - associated vesciles 以上的结果表明类细胞质力蛋白中间链存在于百合花粉及花粉管中，并且在百合花粉管中顺面和反面高尔基体附近的囊泡膜上均有分布。
3.
In animal and lower plant cells , cytoplasmic dynein involved in not only trafficking of subcellular organelles and target molecules , but also organizing and positioning of organelles such as golgi apparatus 细胞质力蛋白在动物和低等植物细胞中不仅参与细胞内细胞器和靶分子等的运输，还参与高尔基体等细胞器的组装和定位。
4.
After treatment with bfa , immunoflurescence labeling and confocal laser scanning microscopy indicated that the cytoplasmic dynein intermediate chain - like protein in lily pollen tube was insensitive to bfa treating , but spectrin - like protein was sensitive to it under the same condition 用bfa处理百合花粉管后，通过免疫荧光标记及激光共聚焦扫描显微镜观察发现类细胞质力蛋白中间链对bfa不敏感；同样的处理发现类红膜肽对bfa敏感。
5.
By sds - page and immuno - blotting , we found that a monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain antibody could react with cytoplasmic dynein intermediate chain - like protein at 67 kda in lily pollen . under confocal laser scanning microscopy after immunoflurescence labeling , we found that the dynein intermediate chain - like protein appeared punctated and was co - localization partly with microtubules in cytoplasm of lily pollen tube 免疫荧光标记及激光共聚焦扫描显微镜观察发现，类细胞质力蛋白中间链在百合花粉管中存在于颗粒状细胞器上；免疫荧光双标及激光共聚焦扫描显微镜观察发现，百合花粉管中类细胞质力蛋白中间链和微管存在部分共分布。
6.
By sds - page and immuno - blotting , the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen . subsequently by immuno - gold labeling and transmission electron microscopy , we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles . statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles 对分离纯化的百合花粉及花粉管中高尔基体组分进行sds -聚丙烯酰胺凝胶电泳和免疫印迹发现，抗鸡脑细胞质力蛋白中间链单克隆抗体在67kda处有较强的免疫交叉反应；进而通过免疫金标结合电子显微镜观察发现，大多数类细胞质力蛋白中间链存在于高尔基体附近的囊泡膜上；统计结果表明，类细胞质力蛋白中间链在顺面和反面高尔基体附近囊泡膜上的分布机率大致相等。
