| 1. | One version of the dideoxy sequencing method can be used to sequence the 5'ends of rna molecules by "primer extension" .
一种形式的双脱氧序列测定法是通过引子的延长来测定RNA分子5’末端的序列。 |
| 2. | One version of the dideoxy sequencing method can be used to sequence the 5 ' ends of rna molecules by " primer extension "
一种形式的双脱氧序列测定法是通过引子的延长来测定rna分子5 ’末端的序列。 |
| 3. | 4 . the seguence of cloned endostatin gene was confirmed with the dideoxy chain - termination method and is consistent with reported sequence of mouse endostatin
4 .以双脱氧末端终止法对重组质粒pbv220一endostatin进行测序，结果表明与己知小鼠endostatin基因序列一致。 |
| 4. | The positive cloned was sequenced by sanger " s dideoxy sequencing method . the results demonstrated the size of hn gene was 1930bp in length and included an open reading frame encoding a protein of 578 amino acid residues
经核苷酸测序，该基因全长1930bp ，含一个1734bp的开放阅读框，编码578个氨基酸，与国外报导的hn基因相比，核苷酸同源性为95 |
| 5. | To investigate the distribution of gene mutations in lpl and to study the relationship between lpl mutations and plasma lipid , the lpl gene ( exon5 ~ 9 including intron - exon boundaries ) was examined by pcr - sscp analysis , and the amplified products showing abnormal pattern on sscp were sequenced using dideoxy - mediated chain - termination
在外显子5中，检出一杂合子突变，经dna序列测定证实为pro ( 207 ) leu突变杂合子，该患者为高甘油三酯血症病人。 |
| 6. | The specific recombinant plasmid was identified by molecular weight , pcr and restriction endonuclease analysis . the results indicated that the resuctant construct contained the gene of interest f . the positive cloned was sequenced by sanger ' s dideoxy sequencing method . the results demonstrated that the f gene included an open reading frame which was 1662bp in length and encoded a protein of 553 amino acid
经核苷酸测序，该基因含一个1662bp的开放阅读框，编码553个氨基酸，与国外报导的新城疫各毒株f基因相比，核苷酸序列同源性为88 . 3 96 . 4 ，氨基酸同源性为92 . 1 96 . 4 。 |
