| 1. | Calmodulin - dependent cyclic nucleotide phophodiesterase was prepared from bovine brain by two - step deae - cellulose column chromatography
摘要通过两次离子交换柱层析，从牛脑中制备钙调素依赖性的环核苷酸磷酸二酯酶。 |
| 2. | 3 . successful purification of the crude ha was achieved by anion exchange resin deae - cellulose column chromatography using 0 . 6mol / lnacl solution as an eluant
3 .采用阴离子树脂deae一纤维素对透明质酸粗品进行纯化，当naci为0 . 6mol / l时，蛋白质含量较低。 |
| 3. | An active metabolite was obtained by purification with precipitated by ethanol , sephadex g - 25 gel , deae - cellulose ion exchange resin and silica gel column chromatography
经乙醇( 95 )沉淀、 sephadexg - 25凝胶层析、 deae -纤维素离子交换层析和硅胶层析纯化，得到抑制黑曲霉生长的单一组分。 |
| 4. | In this optimal condition , p450nor was expressed massively . the expressed product was then purified by deae - cellulose chromatography . the purified expressed protein showed one band in sds - page and the purification attained anticipative purpose
在此条件下，大规模诱导表达重组细胞色素p450nor ，经deae纤维素色谱柱纯化， sds - page分析表明，纯化的目的蛋白基本上为单一谱带，纯化达到预期效果。 |
| 5. | In this paper , by shorting the length of deae - cellulose column and increasing the ph of elution solution , one kind of go isozyme was purified rapidly from green leaves of spanictu brassica parachinensis bailey and vigna unguiculatew . ssp . sesquipedalis ( l )
本文通过一种改进后的方法，即通过缩短deae - cellulose - 52柱的长度，以及提高洗脱液的ph ，可在9h内从菠菜、菜心和豆角绿叶中纯化go同工酶。 |
| 6. | The isolation and purification of dnaase in the earthworm the earthworm dnaase was purified from the tissue extract of earthworm by denaturing the protein with low ph buffer , high temperature , ammonium sulfate precipitation , deae - cellulose ( de52 ) chromatography and ultra - filter membrane
双胸蚓组织中dna酶的分离纯化双胸蚓组织粗提取液经过选择性酸变性、选择性热变性、硫酸按分段盐析、 deae一纤维素（ de52 ）柱层析、超滤膜分级分离后得到一个电泳纯的dna酶。 |
| 7. | Then enzyme was purified with a deae - cellulose ( 5 . 5x50cm ) column , a toyopearl hw - 65 ( 5 . 5 x 50cm ) column and a sephadex g - 200 ( 5 . 5 x 80cm ) column . finally , the enzyme was purified for 10 folds with the recovery of 17 . 4 % . page showed a single band for the purified creatinase
3 、肌酸水解酶的提纯酶在硫酸铵饱和度为40 80之间完全沉淀，先后经过deae - cellulose离子层析柱、 toyopearlhw - 65疏水层析柱、 sephadexg - 200分子筛层析柱层析，最终使酶提纯10倍，最终得率为17 . 4 。 |
| 8. | The xerocomus spadiceus lectin , xsl , was isolated from extracts of fruiting bodies of the mushroom xerocomus spadiceus using a procedure that involved ( nh4 ) 2so4 precipitation , anion exchange chromatography on deae - cellulose , affinity chromatography on affi - gel blue gel , cation exchange chromatography on cm - cellulose , and gel filtration by fast protein liquid chromatography on superdex 75
从砖红绒盖牛肝菌( xerocomusspadiceus )子实体粗提物中，经过deae -纤维素阴离子交换层析、 affi - gelbluegel亲和层析、 cm -纤维素阳离子交换层析和superdex75fplc凝胶过滤，纯化了砖红绒盖牛肝菌凝集素。 |
| 9. | To isolate and purify dnaase in the earthworm first , the tissue extract of earthworm was prepared by dissolving the earthworm with sucrose and denaturing the protein with low ph buffer . then dnaase was purified by denaturing the protein with higher temperature . the following steps were ammonium sulfate precipitation , deae - cellulose ( de52 ) chromatography and filtration by ultra - filter membrane
双胸蚓组织中dna酶的分离纯化采用蔗糖溶解双胸蚓，并选择性酸变性制备双胸蚓组织粗提取液，再经选择性热变性、硫酸铵分段盐析、 deae ?纤维素( de52 )柱层析及超滤膜分级分离对双胸蚓组织中dna酶进行分离纯化。 |
| 10. | The enzyme was purified from cell extract by ammonium sulfate fractionation ( 30 % - 80 % saturation ) and consecutive column chromatography using deae - cellulose and sephadex g - 100 . the sod activity for purified enzyme could reach 4966 iu / mg proteins , and the molecular weight of the sod was about 20 kda which determined by sds - page electrophoresis . when the non - denaturing polyacrylamide gel stained with substrate for sod in the present of 0 . 2mmol / l kcn or 0 . 5 mmol / l h2o2 , the active band was still showed at the same position , which indicated the sod could not be inhibited by the treatments with kcn or h2o2 and it was the mn sod
通过sds一聚丙烯酸胺凝胶电泳可知该sod酶的分子量约为20kda .在非变性聚丙烯酞胺凝胶( pagb )电泳后，在凝胶son显色反应液中加入0 . 2耐01 / lkcn或0 . 5inmol / lh202 ，凝胶sod显色反应后在原来的sod酶部位仍然含有sod活性带，这表明利用kcn和h20 :处理并不能抑制500的活性，该sod属于mn一sod 。 |
