| 1. | We report a case of a 58 - year - old woman having cervical chordoma causing related symptoms for 5 year
我们报告一例58岁女性患有颈椎脊瘤及相关症状五年。 |
| 2. | Discovery and study of novel genes related to chordoma helps to understand the molecular mechanisms better in the development of chordoma
在本研究中我们应用mrna差异显示、 rt - pcr和免疫组化技术对脊索瘤相关基因进行了研究。 |
| 3. | Chordoma , a frequent malignant tumor of bone , is usually adult epithelial tumors arising from embryonic notochord remains of the axial skeleton
脊索瘤是发生于神经轴中线并侵及邻近骨的脊索残留物，是脊柱肿瘤中最常见的一种。 |
| 4. | Chordoma should be included in the differential diagnosis of a dumbbell - shaped mass involving upper cervical vertebrae in patients with long duration of symptoms
若病人在上颈椎呈现哑铃状肿块及患有长期症状时，脊索瘤应列入鉴别诊断中。 |
| 5. | Cloning , sequencing and identifying two novel cdna fragments related to chordoma are described in the first part and expression of fhit gene in chordoma is in the second part
Dd一pcr产物应用凝胶系统分离、扫描，切下差异条带，重新进行pcr扩增纯化，扩增体系、条件同dd一pcr 。将纯化的。 |
| 6. | Materials and methods in this study , we perform 18 cases of chordoma tissue and its normal tissue around them as materials . genomyx fluoro dd kits are from beckman compa - ny , usa
选用通用引物竹和m13对纯化后cdnai和cdnaz进行测序，测序结果大小分别为1045bp和870bp ，与纯化结果一致。 |
| 7. | The first part cloning , sequencing and identifying two novel cdna fragments related to chordoma introduction in this study , we use dd - pcr and rt - pcr to clone , sequence and i - dentify genes related to chordoma
我们选择其中9条cdna差异带用于分析，其中cdnai和cdnaz的大小分别为1 ， 4kb和85obp左右。纯化后的两个cdna差异片段与所切差异条带大小一致。 |
| 8. | Many published papers discussed its clinic characteristic points , but little is known a - bout the molecular changes in this disease . few protooncogenes and tumor sup - presser genes have been found to be altered in chordoma
因而脊索瘤相关基因研究将有助于脊索瘤基因诊断和基因治疗的进展，从而在分子机制上彻底去除或阻断致病基因的作用，达到治疗脊索瘤的目的。 |
