Comparative study on the characteristics of immune response induced by 97kda dna and uv - attenuated cercariae vaccines of schistosoma japonicum 疫苗与致弱尾蚴疫苗诱导免疫应答特征的比较研究
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Observation of effects of 11 kinds of traditional chinese herbal medicine to prevent the cercaria of schistosoma japonicum from entering cutis 试论开展突发公共卫生事件症状监测的必要性和困难
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G / dose / mouse ) . eight weeks after final boosting , vaccinated and control mice were challenged percutaneously with 40 ? 1 s . japonicum cercariae ( chinese mainland strain ) by the cover glass method 单独np3o组：仅以np3ozo以g鼠免疫小鼠，免疫时间、次数均与ca np30组相同。
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Cercariae were collected , cultured in vitro and transformed to schistosomula in the rpmi 1640 medium with rabbit serum . the schistosomula were cultured in conditional media up to 96 hours . the number of schistosomula was counted and the death rate was calculated 将条件培养基与童虫共培养， 96h内连续观察计数，计算童虫死亡率，与阴性对照比较，童虫死亡率最高的基因池进入下一轮筛选。
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Researches of schistosomiasis vaccines have gone more than 60 years , approximately including from the stages of dead vaccine and live vaccine ( irradiated attenuated cercariae vaccine ) to gene engineered vaccine , etc . many different forms of vaccines have been tested in animal models , including gluthathione s - transferase , paramyosin , irv - 5 , triose phosphate isomerase , sm23 , fatty acid binding protein ; which were considered promising by who / tdr . but none of them steadily accomplished the pre - set target level of 40 % protection . in order to enhance the protective capacity further , it is essential to develop novel vaccine antigens and / or vaccine adjuvants 血吸虫病疫苗研究已有60多年的历史，大致经历了死疫苗、活疫苗(照射致弱尾蚴疫苗)和基因工程疫苗等研究阶段，产生了一些who / tdr推荐认为很有希望的疫苗候选分子，如谷胱甘肽- s -转移酶( gst ) 、副肌球蛋白( sm97 ) 、照射致弱疫苗抗原5 ( irv - 5 ) 、磷酸丙糖异构酶( tpi ) 、曼氏血吸虫膜内在蛋白( sm23 )和脂肪酸结合蛋白( fabp ， sm14 )等，但其对宿主的保护作用均不甚理想，未能稳定地达到40或以上的保护力水平，因此有必要继续寻找新的疫苗抗原分子和/或疫苗佐剂，进一步提高其保护力。
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The mouse model of hepatic egg granuloma of schistosoma japonicum confirmed that np30 has sensitizing effect on formation of hepatic egg granuloma , active immunity in sheeps and mice with np30 can induce good protecti on against infection with schistosoma cercariae and reduces immunopathologic damage to host , np30 plays a significant down - modulatory role to hepatic granuloma and fibrosis 以往的研究业已证明np30对虫卯肉芽肿的形成具有致敏作用，并有十分显著抗虫卵肉芽肿肝纤维化作用，用np30主动免疫小鼠、山羊可减轻日本血吸虫对宿主造成的免疫病理损害。
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Yherefore , many researchers have shown great concern with the development of vaccines to against schistosomiasis . at early stage , the research of vaccines of schitosomiasis was centered on dead vaccine and athenuated cercaria of schistosomes while molecular vaccine and athenuated cercaria of schistosomes while molecular vaccine is currently the focus of research with anti - infection protective immunity as its main concern . guan xiaohong and zhao weixian ( 1986 ) certified that the allergen of egg granulonma of schistosoma japonicum might firstly come from gut associated antigen ( gaa ) of schistosomula and adult worm and that daa had cross reaction with soluble egg antigen ( sea ) and membrane associated antigen ( maa ) ; and the gaa of schistosoma japonicum might play a sensitizing role in egg granuloma formation Np30主动免疫c57bl 6对尾蚴攻击感染产生42 . 05的保护力，肝组织减卵率为66 . 63 ； balb c和昆明种小鼠的保护率分别为39 . 53和50 . 46 ；免疫山羊可诱导42 . 78的减虫率，肝组织减卵率为35 . 83 ，粪减卵率为25 ，并可明显抑制肝脏虫卵肉芽肿的大小，肉芽肿数量明显减少，纤维化减轻，体重明显增加，因此np30是南京医科大学博士学位论文很有希望的抗日本血吸虫病疫苗侯选分子。
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The target gene was then subcloned into plasmid vector and induced by iptg for its expression . after that , the recombinant protein was purified and used for the identification and characterization of its immunogenicity . the effect of the induced specific ige antibody on the hepatic granuloma formation and fibrosis after challenge infection with schistosome cercariae was evaluated Niptg诱导表达，产物沉淀中于约45kda处显见高合蛋白表达带， westernblot分析表明，该蛋白带可被筛库血清中特异性lge抗体识别；而载体本身表达的26gst蛋白带则否。
