| 1. | Cloning and expression of the rabbit growth hormone cdna 兔生长激素cdna克隆和表达 |
| 2. | Rabbit growth hormone ; cdna clone ; expression 兔生长激素cdna克隆表达 |
| 3. | Cdna clone and lignite degradation ability of lignin peroxidase from 斜卧青霉p6木素过氧化物酶的褐煤降解活性及cdna克隆 |
| 4. | Cdna clone and lignite degradation ability of lignin peroxidase from penicillium decumbens p6 斜卧青霉p6木素过氧化物酶的褐煤降解活性及cdna克隆 |
| 5. | Cdna cloning , sequence analysis , expression and protein structure modeling of hla - g1 from chinese 中国人hla - g1的cdna克隆序列分析表达和蛋白质空间结构预测 |
| 6. | The plasmid ploxgfp dna was transfected into bovine fetal fibroblast cells , then on the selected g418 medium , the green fluorescent cell clones were gained 将鸡-干扰素cdna克隆到载体plox上的loxp和lox511序列之间,构建成另一打靶载体? ploxifn 。 |
| 7. | The cdna of preprotein and mature protein of rabbit hypophysiscerebri was obtained . the cdna of mature growth hormone was cloned in pet - 3a , then the recombinant expression vector was transformed into 从兔脑下垂体中的mrna中,利用合成引物逆转录pcr ,获得了前体和成熟生长激素的cdna ,成熟生长激素的cdna克隆在pet - 3a表达载体并转化到大肠杆菌bl21 de3 plys中,获得了高效表达,表达量达到大肠杆菌总蛋白的40 % 。 |
| 8. | Finally , the full length cdna of real differential fragments were cloned by performing both 5 ' - and 3 ' - rapid amplification of cdna ends ( race ) and then the functional screening was performed by transferring the full length cdna into arabidopsis thaliana or nicotiana tabacum plant 108号克隆尚未做测序分析。通过race技术对152和233两个差异片段进行全长cdna克隆,获得了152的全长cdna序列和233的3 ’ 。 cdna序列。 |
| 9. | The deduced n - terminal 76 amino acids of the protein were 100 % identical to those of abstract human and rat ubiquitin ( ub ) , and the carboxyl extension protein ( cep ) sequences were also highly similar to those of the homologous proteins of human , rat and other species 我们将这个cdna克隆命名为amphiubf80 。 089cdna序列编码一个188个氨基酸残基组成的蛋白质,与人aoeb166蛋白( prxv )的同源性达65 ,我们将这个cdna克隆命名为amphiprxv 。 |
| 10. | This understand of stored nitrogen compounds restricted seriously the progress in the investigation of vegetative storage proteins . in the dissertation , we studied more extensively the cytology , biochemical properties and biological roles of vegetative storage proteins in swietenia macrophylla and in hevea brasiliensis by light - and electron microscopy , sds - page , page , immuno - blotting , indirect immunohistochemical localization and colloidal gold labelling and cdna clone techniques 采用光镜和电镜技术、 page 、 sds - page和免疫印迹技术、电泳凝胶过碘酸? schiff试剂染色、间接免疫荧光和电镜免疫细胞化学定位技术以及cdna克隆技术,较深入地研究了大叶桃花心木和巴西橡胶树的营养贮藏蛋白质的细胞学、生物化学性质和生物学功能。 |