2 . the primary culture of silkworm embryonic cells ( 1 ) this dissertation took silkworm blastokinesis embryo as the materials of primary culture and found a better method of fragment embryo to primary culture by comparing the methods of fragment embryo that mincing embryo by scissors and digest embryo by trypsin after mincinging . that is the latter 2家蚕胚胎细胞的原代培养门）本研究以家蚕反转期胚胎为材料，采用组织块法和胰蛋白酶半消化法进行家蚕胚胎细胞的原代培养，建立了适应反转期胚胎细胞原代培养的方法，即胰蛋白酶半消化法。
