| 1. | Bacterial lipase is a kind of biocatalyst which is widely used in many industrial fields
摘要细菌脂肪酶是一类广泛应用于工业领域的生物催化剂，具有重要的应用价值。 |
| 2. | The unconventional solvent properties of ionic liquids have been exploited in biocatalyst recycling and product recovery schemes
目前离子液体的溶解特性已在生物催化再循环和产品的回收方面得到了很好的应用。 |
| 3. | What enzyme / organism was used as the biocatalyst and how was it selected ? for a purified enzyme process , include the source organism for the enzyme
什么样的酵素/生物被用来做为生物触媒，且如何选择?对于纯化酵素的程序，则包括酵素的来源种属。 |
| 4. | The result of using eight kinds of different yeast strain as biocatalyst reveals that baker ' s yeast is the best biocatalyst in this transformation
比较了八种酵母的生物转化能力，发现在有机合成生物转化中应用最广泛的生物催化剂baker ' s酵母最适合催化该模型反应； 3 |
| 5. | Immobilized enzyme has been applied to numerous fields , including bioreactors , biocatalysts and biosensors because of its advantage such as good stability and reusability
固定化酶具有稳定性好，使用次数多等独特优点，已被用于生物反应器，生物催化，和生物传感器等众多领域。 |
| 6. | The lipase b from candida antarctica ( cal - b ) displays high enantioselectivity on a broad range of substrates , making it an accepted biocatalyst for asymmetric organic chemistry
摘要从南极假丝酵母中提取的脂肪酶b ，具有较高的对应异构体选择性，广泛应用在培养基中，作为不对称有机化学的生物催化剂。 |
| 7. | During down processing biocatalyst several immobilization method are discussed and the result reveals that the method with alginate sodium as entrapment and cach as cross - linking reagent is best for the conversion , and the concentrate of those are 3 % and 0 . 6mol / l
进一步的研究表明，海藻酸钠为3 (质量浓度) 、氯化钙为0 . 6mol / l的情况下最有利于模型反应的进行。 |
| 8. | E . coli xl1 - blue cells were tansformed by psurfpga and phages were rescued by m13ko7 helper phage particles . results showed that the heterodimeric enzyme was expressed as a fusion protein that matures to an active biocatalyst connected to the coat protein of phage fd
以构建的噬菌粒psurfpga转化具有琥珀突变的大肠杆菌xl1 - blue ，以辅助噬菌体m13k07超感染，进行青霉素g酰化酶基因的表达和在噬菌体表面的展示。 |
| 9. | Study of " ph memory " and activity water ( aw ) in processing of immobilization indicates that former factor has much effect on transformation and the latter just has little effect . permeabilization treating of baker ' s cell with alcohol and ultrasonic leads to lower transformation rate , which illustrates that permeabilization procedure has negative role on biotransformation with whole cell as biocatalyst in organic solvent . in simple dynamics research amount of biocatalysu concentration of substrate and reaction course are considered and all of them have remarkable effect on transformation
Ph记忆、水活度及通透性处理的实验结果表明， ph记忆对转化有明显的影响，水活度对模型反应的影响不大；作为完整细胞生物催化，细胞的通透性可能是传质阻力的重要组成部分，利用不同浓度的乙醇和超声波对细胞进行处理，结果表明，在有机相完整细胞生物催化中，通透性处理对转化产生的是负面影响。 |
| 10. | Biotransformation in organic solvent is an attractived field in nowadays . compared with isolated enzyme whole cell is not used broadly as biocatalyst . in this research the cells of baker ' s yeast is adopted to mediated a model reaction in organic solvent , in which geraniol is converted to citronellol reductively
有机相生物转化是当前生物技术中一个具有理论研究意义和应用价值的领域，目前该领域的研究大多集中在利用分离的酶进行生物催化，利用微生物完整细胞进行的研究比较少。 |
