Cultivation of auricularia auricula in logs 段木栽培黑木耳技术
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Assessment the genetic diversity of auricularia strains by two pcr - based typing methods 方法对木耳属菌株的遗传多样性评价
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B . take more edible fungus auricularia auricula - judae , mushroom , walnut and vegetable , etc 2多食木耳蘑菇核桃蔬菜类。
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This paper stated productive technology , productive modes , productive equipments and technical requirement and managemental patterns of auricularia auricular with full - light cultivation 摘要本文对北方黑木耳的生产工艺、生产方式、生产设备、技术要求、全光栽培管理方式等要点进行了阐述。
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The strain 2009 and the pure culture were used as experimental materials to study the relationship between the pregermination methods of auricularia auricula and the emergence rate , the contamination rate and the yield by six methods 摘要以黑木耳菌株2009作试验菌株，选用木屑培养基作试脸材料，在同一黑木耳栽培菌培养室采用6种不同的催芽方法，研究了催芽处理方法与耳基分化率、污染率和产量之间的关系。
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In this study , 29 strains from 3 auricularia species were chosen for genetic identification and genetic diversity assessment by using methods such as morphological taxonomy , isozyme techniques , rapd analysis , eric - pcr and its - rflp 本研究以木耳属3个种29个菌株为供试材料，通过形态学分类法、同工酶技术、 rapd方法、 eric - pcr以及its - rflp技术，对木耳属种质资源进行遗传鉴定和遗传多样性评价，结论如下： 1
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It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent , genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study , genetic diversity in a . polytricha was higher than that in a . auricula : 4 in this study , a . fuscosuccinea had a higher homology to a . auricula than to a . polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a . auricular and a . fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a . auricula as a probe which hybridized with a . auricula and a . fuscosuccinea except a . polytricha , further confirming the veracity of the results from eric - pcr ; 7 in this study , isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity 本研究中，木耳属2个种的2个菌株在its区域表现出较高的保守性， 4种限制型内切酶的酶切图谱没有显示出多态性；增加内切酶种类及供试菌株数量，有可能获得具有多态性的限制性内切酶酶切图谱； 9本实验中， its区域的真菌特异性引物与真核生物通用引物对于扩增效果无较大差异，扩增片段长度均为650bp左右； 10根据形态学实验、 eric - pcr实验以及southern杂交实验的结果分析，紫木木耳属种质资源的遗传鉴定和遗传多样性评价耳极有可能是毛木耳种的一个变种; n .本研究中所用的gutc法是一种适用于木耳属菌株基因组洲a快速提取的方法; 12 .传统的形态学分类法和现代的分子生物学分类法，两者的关系是相辅相成，互为验证
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The second part has first selected the best formula of mother and stock culture medium which adapt to pleurotus ostreatus , lentinus edodes , flammulina velutipes , agrocybe cylindracea , volvariella volvacea , agaricus bisporus , auricularia auricula , pleurotus membraneens , ganoderna lucidium and agaricus blazei 第二部分：通过一系列的培养生态试验，初步筛选出了适宜平菇、香菇、金针菇、杨树菇、草菇、双孢蘑菇、木耳、凤尾菇、灵芝和巴西蘑菇等10种食用菌的母种培养基配方和原(栽培)种的培养基配方。
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The results are as following : 1 eric - pcr was for the first time applied in differentiating strains from edible fungi and proved to be more rapid and reliable than rapd in auricularia identification study . taken the similarity coefficient as 75 % , 29 strains of three auricularia species were grouped into 6 and 9 clusters by rapd and eric , respectively . eric - pcr clearly distinguished a . auricula from a . polytricha while rapd failed 在75的分类水平上， eric - pcr把29个菌株分为9组，黑木耳种和毛木耳种可以明显区分开；而rapd只能分为6组，不能将黑木耳和毛木耳分开，说明eric - pcr是比rapd更快捷可靠的分子标记，可以有效用于木耳属的种质资源及遗传分类的研究，也适合于其它食药用菌种质资源的研究； 2
