Aligned with cytochrome b sequences from 29 siluriforms in japan , korea , russia , africa , north america and south america retrieved from genbank , and selected astyanax mexicanus , phenacogrammus interruptus , cyprinus carpio and oncorhynchus mykiss as outgroups , which are belonging to characiformes , cypriniformes and salmoniformes , respectively . we constructed a matrix of 65 dma sequences . the kimura ' s two - parameter distances were calculated and molecular phylogenetic trees were constructed by using the maximum parsimony ( mp ) , maximum likelihood ( ml ) and neighbor - joining ( nj ) methods 所得序列与从genbank下载的分布于非洲、北美洲和南美洲等地的鲇形目鱼类同一基因序列进行排序，并选用脂鲤目( characiformes )的墨西哥丽脂鲤( astyanaxmexicanus ) 、断线脂鲤( phenacogrammusinterruptus ) ，鲤形目( cypriniformes )的鲤( cyprinuscarpio )以及鲑形目( salmoniformes )大麻哈鱼属的oncorhynchusmykiss作外类群。
