Haemostaseology - determination of the antithrombin iii activity - part 1 : reference measurement procedure using a synthetic peptide substrate 止血学.抗凝血酶iii活性的测定.第1部分:用合成肽底物
2.
Value of plasma thrombin - antithrombin complexity and alpha granule membrane protein in patients with acute myocardial infarction treated by thrombolytic therapy 140在急性心肌梗塞患者溶栓治疗中的应用价值
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Amidolytic assay of thrombin approved that the recombinant haemadin does have the antithrombin activity . its inhibition constant 通过以chromozym th为底物的凝血酶酰胺水解实验证实了其体外生物学活性：其抑制凝血酶常数为2 . 460 . 13 10
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Heparin is known to bind and modulate the activity of various proteins , including cell growth factors , cytokines , angiogenic factors , complement components , adhesion molecules , heparin exerts its activities via interaction with some proteins . potent anticoagulant activity of heparin by the interaction with antithrombin iii is extensively used in medicine 肝素能够与许多蛋白质结合并调节蛋白活性，包括细胞因子、生长因子、趋化因子、抗血管生成因子、粘附分子和补体成分等，肝素通过与蛋白相互作用发挥其生物学活性。
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Strain bl21 , and gene expression was induced by iptg . the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene . using ion exchange chromatography and gel filtration chromatography , the chimera proteins were purified , and both of them showed a single band in tricine - sds - page . the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities , but gain platelet aggregation inhibitory activities as well 通过离子交换层析和凝胶过滤层分别对两种嵌合体蛋白进行纯化，纯化产物在tricine - sds - page中都显示为单一条带。活性分析结果表明两种嵌合体蛋白在保留水蛭素抗凝血酶活力的同时，还呈现抗血小板聚集活性。
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A method for preparation of antithrombin ( at - ) concentrated from human plasma ' s fraction has been described . after preliminary treatment of plasma with the cold ethanol , the isolation of at - iii from the precipitate was performed by affinity chromatography on heparin - sepharose cl - 6b ; desalted by dialysis and concentrated 从人体血浆中提取较高纯度的抗凝血酶( at - )的，通过低温乙醇处理血浆，得到的沉淀经肝素-琼脂糖两次亲和层析，并透析、浓缩获得at -的初品。
