| 1. | Study on elisa method to detect the antibody of aev
酶联免疫吸附试验检测禽脑脊髓炎抗体方法的研究 |
| 2. | The purpose of this study was to learn the sequence and structure of the aev by technology of the molecular biology . to reveal the relation between genome features and function
本项研究，旨在利用分子生物学的技术手段，搞清楚aev基因组的序列和结构，从而探讨基因结构与功能的关系。 |
| 3. | It was firstly reported in china . the third part rthe capsid protein vp | gene was expressed . 810 bp fragment was inserted into vector pgex - 4tl , and then transformed into e . coli bl21
这是国内首次对aev基因组全序列的分析；第三部分： aev外壳蛋白vp _ 1基因在大肠杆菌中的融合表达、纯化及活性研究。 |
| 4. | Synthesis of igm was restrained after igg was produced , because igm antibodies were producld first in an immune response against aev followed by a switch to igg class
当igg出现后， igm的合成即受到抑制。抗aev的血清igm和igg在时间上和强度上出现双峰现象， igm和igg的产生是同一b细胞克隆类型转换造成的。 |
| 5. | Aev has long been recognised as the aetidaogical agent of one of the most significant disease of poultry . despite its importance , relatively little progress had been made towards an understanding of its basic biology and pathogenesis
Aev一直被认为是最重要的禽病病原之一，尽管它很重要，但有关其基础生物学，发病机理的了解却较少。 |
| 6. | 10 . 14 . 25 and 30 days post injection and separated the serum . meanwhile , pathological changes of lymphocytes and changing role of igm , igg from different days old chickens infected with aev - nh937 were systematically studied by viral inoculation of animal , pathological test and elisa
采用了病毒接种技术、普通病理学方法、酶联免疫吸附实验等方法，研究了不同日龄spf雏鸡人工感染aev - nh937株后的血清igm 、 igg的动态变化规律。 |
| 7. | When artificially and naturally infected , antibody produced is mainly igg , which with high content and long endurence . igg has multiactivities of antibacterial , antivirus , antiexotoxin and so on . it was proved that humoral immunity play important role against the aev - nh937 strain infection at the beginning of the disease
在人工自动免疫和自然感染后，机体所产生的抗体主要是igg ，它不仅含量多，而且持续时间长，是抗感染免疫的主力，具有抗菌、抗病毒和抗外毒素等多种活性。 |
| 8. | Avian encephamyelitis ( ae ) , which is also called epidemic tremor , characterized by nonsuppurative encephamyelitis , is caused by avian encephalomyelitis virus and has a sign of decreasing egg production . in adult chickens and decreasing hatching rate in fertilized eggs and has 40 % - 60 % of incidence and 25 % of mortality
该病发病率为40 60 ，死亡率为25左右。成年鸡感染后出现产蛋率下降，种蛋出雏率降低。但雏鸡感染aev的早期血清中的igm ， igg系统的变化规律尚未见报道。 |
| 9. | Rt - pcr was used to amplify the cdna of the genome . these cdna fragments were cloned into the plasmid pucm - t . the result indicated that the seguence of the genome was obtained . the genome of aev - nh937 composed of 7055 nucleotides , potentially encodes a polyprotein of 2134 amino acids . the genome of aev - nh937 has 94 . 3 % nucleotides identity with the calnek vaccine strain of aev
把它们分段克隆在pucm - t载体上，经序列分析，获得了aev - nh937毒株的基因组全序列及推导的氨基酸序列，基因组全长为7055个核苷酸，与calnek疫苗株具有94 . 3的同源性，编码一个含2134个氨基酸的多聚蛋白。 |
| 10. | The research consist of four parts . the first part is multiplication , purification and electron microscope examination of the avian encephalomyelitis virus . a 1 : 5 dilution of isolate - nh937 of aev and control group of pbs were inoculated to susceptible 6 - day - old chickens embryos . respectively . after incubation for 10 days , the urinay vesicle liquid was collected . making a comparison the size of the chickens embryos between the test group and the control group , the results showed that the size of the control group is bigger than that of the test group . purified virions were examined under the electron microscope , the result revealed that there are a lot of virions and the aev - nh937 was multiplicated in embryos . the second part was seguence analysis of the genome of the aev - nh937 . nine pairs of primers were designed according to published calnek vaccine strain of aev
本研究共分四个部分：第一部分为aev的增殖，纯化和电镜观察，用1 : 5倍稀释的aev - nh937株和阴性对照pbs分别经卵黄囊接种于6dspf鸡胚，继续孵化10d后，收集尿囊液。比较接种组和健康对照组鸡胚的大小，结果显示，健康对照组鸡胚明显大于接种组。分离、提纯aev ，把纯化的病毒在电镜下观察，证明确有大量aev病毒粒子存在，说明aev在鸡胚中成功扩增；第二部分是aev - nh937基因组的序列测定工作。 |
