负链 meaning in Chinese
minus strand
negative strand
negative-sense strand
Examples
- Group v : ssrna viruses
负链rna病毒 - The causative agent of the disease , newcastle disease virus ( ndv ) , is a member of the paramyxoviridae whose genome is a non - segmented single - strand negative - sense rna
该病的病原新城疫病毒( newcastlediseasevirus , ndv )属副粘病毒科，为有囊膜的负链rna病毒。 - After sequencing and cutting vector sequences by vecscreen software , these fragments were registered directly on - line and eight gi numbers were given : ay247106 , ay220755 , ay220754 , ay216525 , ay216526 , ay220756 , ay220753 , ay220752 , which are corresponding to zhyf001 ~ zhyf008 respectively . these dna fragments were aligned by the software , vector nti 6 . 0 , with neighbor joining ( nt ) method
7和100 ； zhyf001 zhyf005基因序列的正链和负链均与上游弓物序列100配对，但找不到下游引物序列的互补序列； zhyf008序列与上游引物序列100配对，但也找不到与下游引物序列的互补序列。 - Then , the plasmid was transformed into jm109 . the full length pstvd cdna recombinant plasmid was further identified by restriction mapping analysis and the nucleotide sequence of cdna cloned in pmd 18 - t vector was analyzed with ab1 377 dna sequence . test showed that the cdna of this chinese pstvd isolate was the same as pstvd - kf - 6 mild " type " isolate which originated from naturally infected potatoes in field of cornell university potato breeding program
利用rt - pgr技术，设计一对引物，对田间采集的经鉴定含pstvd的阳性样品进行全序列扩增，并将所得产物纯化回收，连接到pmd18t - vector中，并转化至大肠杆菌jm109中，挑选白斑进行双酶切( saci / ecorv )鉴定证明插入片段为359bp大小，进行序列测定，所得克隆基因为pstvd全序列负链，大小为359bp 。