乳链菌肽 meaning in Chinese
nisin
Examples
- Optimization of agar diffusion bioassay for nisin
琼脂扩散法测定乳链菌肽效价的优化 - The progress of quantification and fermentation of nisin
乳链菌肽检测与发酵技术研究进展 - A dna fragment was amplified from lactococcus lactis nizo r5 genome by means of pcr and cloned into pmg36e plasmid . the recombinant plasmid was transformd into e . colijm 109 and identified by restriction endonucleases analyzing . the recombinant plasmid was introduced into lactococcus lactis nz9800
本实验提取乳酸乳球菌nizor5的基因组dna为模板，用pcr方法得到乳链菌肽前体基因( nisa ) ，克隆到乳酸乳球菌表达载体pmg36e ，构建了表达载体pmg36e nisa 。 - A approximately 460bp dna fragment was amplified by pcr from lactococcus lactis nizo r5 genome . the primers used in this study comprised the following nucleotide sequences : 5 " - cgcgaattcgatataggtttattgagt - 3 " and 5 " - atgaagcttatccatgtcagaactaa - 3 " . conditions used for the pcr consisted of 30 cycles of 94 ? for0 . 5min , 45 ? for imin and 72 ? for 1 . 5min , plus one additional cycle of 72 for 10min
根据已发表的乳链菌肽前体基因的dna序列，设计两条引物并引入酶切位点。以lactococcuslactisr5基因组dna为模板， pcr反应条件： 94变性30s ， 45退火60s ， 72延伸90s ，反应进行30个循环。成功扩出一条约460bp的dna片段。