前导肽 meaning in Chinese
leader peptide
Examples
- On the other hand , dnastar software was utilized in the analysis of the different segments of bdnf genes . the reasonable phylogenic tree was constructed with the leading peptide encoding sequences
运用软件dnastar中的megalign程序分别对28种动物bdnf基因的不同区段进行分析，发现采用前导肽区编码序列能够得到比较正确的系统树。 - The scfv genes were then inserted into the prokaryotic fusion protein expression vector ptat - ha and expressed in e . coli bl21 . after the expression products were purified by ni - nta colume , their activities were analysed
将获得的不含前导肽的单链抗体基因克隆入ha和6his融合的原核表达载体ptat - ha ，在大肠杆菌bl21中进行表达，分析其表达情况。 - Due to high homology of nucleotide between ba - dfe and subtilisin bpn , primers were designed and synthesized . the intact ba - dfe gene was amplified by pcr and cloned . the sequence analysis indicated that the ba - dfe gene has an open reading frame with 1146bp , which encodes 382 amino acid residues containing signal peptide , pro - peptide and mature peptide
序列分析显示，该片段的核昔酸序列中含有1146hp的开放阅读框，可编码382个氨基酸残基的ba dfe前体蛋白，包括30个氨基酸残基组成的信号肽、 77个氨基酸组成的前导肽和275个氨基酸残基组成的成熟肽。 - Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library . the products were cloned into puc19 vector and their sequences were analysed . the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region , with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv
方法以从噬菌体抗体库中筛选获得的抗hbsag的fab抗体基因为模板，分别扩增出其轻、重链可变区( v _ l 、 v _ h )基因，通过重组pcr方法将轻、重链可变区基因用连接肽( gly _ 4ser ) _ 3的编码序列连接，并引入前导肽编码序列，构建具有l - v _ h - linker - v _ l结构的单链抗体基因。