| 1. | These results suggested that the contents of 5 - ht and its receptor may implicate mature development of rat
这提示大鼠颌下腺的5 -羟色胺及其受体含量可能与其发育成熟有关。 |
| 2. | The smg cell of rats were isolated and purified by pancreation digestin and then were cultured and subculfured in dmem with 20 % fetal bovine serum
应用胰酶消化法进行颌下腺细胞的分离、纯化及原代培养、传代培养。 |
| 3. | It showed that they increased with time passing by during postnatal 60 days and kept steadily from 60th to 90th day
结果发现，大鼠颌下腺5 -羟色胺及其受体的相对含量在生后1 - 60日龄内随日龄的增长而增多，在60 - 90日龄内基本保持恒定。 |
| 4. | After administrated for successive 10 days , mice were anesthetized by sodium phenobarbital , carried out heart perfusion 30min with 2 % paraformaldehyde , then extracted hippocampus and submaxillary gland , made frozen sections
连续灌胃给药10天后，苯巴比妥钠麻醉小鼠，用2多聚甲醛心脏灌流固定30ndn ，取出海马和颌下腺，做冰冻切片。 |
| 5. | The relationship between the relative contents of 5 - hydroxytryptamime ( 5 - ht ) and its receptor and age in days of postnatal rat was studied by using immunohistchemistry and in situ quantitative analysis
为研究大鼠颌下腺5 -羟色胺及其受体的相对含量与日龄的关系，用免疫组织化学法结合原位定量，测定了大鼠颌下腺5 -羟色胺及其受体免疫反应产物的相对含量。 |
| 6. | If a mouse did n ' t reach termination within 2 min , the time was assigned a score of 120s . 3 . the effect of xas on ache in hippocampus and ngf in sub - maxillary gland mice were randomly grouped into blank control , xas groups
文冠果皂贰对小鼠海马内胆碱酯酶（ ache ）和颌下腺内神经生长因子（ ngf ）活性的影响将昆明种小鼠随机分为空白对照组、文冠果皂贰不同剂量组。 |
| 7. | So we observed the effect of xas on ability of learning and memory in normal mice and in mice with memory deficits , and detected the activity of ache in hippocampus and ngf in submaxillary gland to discuss mechanism of action of this medicine
为此，我们观察了文冠果皂甙对正常小鼠及记忆障碍模型小鼠的学习记忆功能的影响，并检测了海马内胆碱酯酶和颌下腺内神经生长因子的活性，探讨该药作用机理。 |
| 8. | Objective as one of salivary glands , submandibulary gland plays an important role not only in the physiological process , but also in the pathological research . now more and more researchers are interested in its theraputiz value of treatment
实验目的颌下腺( submandibulargland ， smg )作为人体三大唾液腺之一，不仅在人体的正常生理活动中发挥着不可缺少的作用，而且在疾病研究方面具有重要的价值。 |
| 9. | In this experiment research , our objective is to study the biocompatibility of the smg cells and collagen sponges . submandibular gland cells of rats were isolated with parcreation digection , and the cultured , cells were subcutaneously injected into collagen sponge
国内学者已经在颌下腺细胞的培养及扩增，生物支架材料的选取及开发方面作出初步探索，而关于颌下腺细胞与支架材料的相互作用方面尚无人问津。 |
| 10. | By the compounds of submandibular gland cells and collagen sponges . we investigate the optimal cell denisity of tissue engineered compound of submandibular gland cells and collagen sponges , the cellular compatibility of tissue engineered compound of submandibular gland cells on the collagen sponges with different porosity and the influence of epidermal growth factor on the adherence of submandibular gland cell to collagen sponge . our studies can primary provide theoretical ground work to form the model in vitro of tissue engineering smg
在本研究中，以初步探讨体外颌下腺细胞与胶原海绵支架相互作用为目的，采用体外分离培养sd大鼠颌下腺细胞，然后接种于胶原海绵支架上体外复合培养的方法；从不同接种细胞浓度对细胞一支架复合物影响，同一接种细胞浓度在不同孔隙率的支架上黏附、增殖的情况及表皮生长因子( egf )对颌下腺细胞的促增殖作用，促细胞在支架上黏附等三方面入手，初步研究了颌下腺细胞与胶原海绵相互作用的影响因素，为进一步在体外及体内构建较为理想的组织工程化颌下腺提供理论参数和实验依据。 |
