English translation for "青霉素g"
|
- pcg
pen-a-brasive
penicillin g
Related Translations:
青霉素类青霉素: penicillin benzylpenicillin 青霉素休克: penicillin shockshock caused by penicillin 呋苄青霉素: furbenifillin (bl-p1597) 羟苄青霉素: carbenicillin indanyl esterr 异噻唑青霉素: isothiazolymethylpenicillin 青霉素v钙: penicillin v calcium 苄青霉素钙: calcium bilirubinate stonecalcium penicillin g
- Example Sentences:
| 1. | Method for the determination of penicillin g , penicillin v , nafcillin , oxacillin , cloxacillin , dicloxacillin residues in honey - lc - ms - ms method
蜂蜜中青霉素g青霉素v乙氧萘青霉素苯唑青霉素邻氯青霉素双氯青霉素残留量的测定方法液相色谱-串联质谱法 | | 2. | E . coli xl1 - blue cells were tansformed by psurfpga and phages were rescued by m13ko7 helper phage particles . results showed that the heterodimeric enzyme was expressed as a fusion protein that matures to an active biocatalyst connected to the coat protein of phage fd
以构建的噬菌粒psurfpga转化具有琥珀突变的大肠杆菌xl1 - blue ，以辅助噬菌体m13k07超感染，进行青霉素g酰化酶基因的表达和在噬菌体表面的展示。 | | 3. | Although some pga precursors formed inclusion bodies in the cytoplasm of dh5a / pkkfpga , the amount only made up 5 - 10 % of all the afpga peptides , this explained why the expression level of afpga in dh5 / pkkfpga was a little lower than that in dh5 / psmlfpga
虽然青霉素g酰化酶在菌株dh5 pkkfpga中形成包涵体，但其量只占总酶的5 - 10 ，解释了为什么菌株dh5 pkkfpga的表达量虽然比菌株dh5 pkkfpga低，但差距不大。 | | 4. | A polypeptide with sequence of qkvdssggggs was designed to be a linker between c terminal of penicillin g acylase and n terminal of the coat protein . the ribosome binding site ( rbs sequence ) of psurfscript is also replaced by rbs sequence originating from bacillus subtilis . it was demonstrated that constructed phagemid can still express penicillin g acylase
将包含信号肽和琥珀终止密码子uag ( amber )的完整巨大芽孢杆菌青霉素g酰化酶基因克隆到噬菌粒psurfscript ，通过引入的11肽连接青霉素g酰化酶的c末端与噬菌体外壳蛋白gp3的n末端。 | | 5. | The purified enzyme had a specific activity of 68 . 6 u / mg protein . overproduction of pga was often limited by translocation and / or periplasmic processing steps , subsequently resulted in intracellular accumulation of various types of pga precursors and then formed inclusion bodies in the cytoplasm and / or periplasm
经deae - sepharosecl6b离子交换层析和butyl - sepharosecl4b疏水层析，即可得纯度提高20倍、比活为68 . 6u mg的青霉素g酰化酶，两步纯化的总得率达91 。 | | 6. | The supernatant fraction and the precipitation fractions were analyzed by western blotfor strain dh5 a / pkkfpga , 5 - 10 % pga precursors formed as inclusion bodies in the cytoplasm while no inclusion bodies formed in the periplasm , this suggested most pga precursors were transported to the periplasm and matured to active pga and indicated that the maturation of pga in strain dh5 / pkkfpga was limited by the translocation step
Western印迹分析表明对于菌株dh5 pkkfpga ， 5 - 10的原前体青霉g素酰化酶在胞内形成了包涵体，说明其成熟的限速步骤在胞内的运输阶段，而菌株dh5 psmlfpga则无明显包涵体形成，说明菌株dh5 psmlfpga改善了青霉素g酰化酶的合成流，因而其表达能力高于菌株dh5 pkkfpga 。 | | 7. | Considering alcaligenes faecalis pencillin g acylase ( afpga ) , which possesses the attractive characteristics for beta - lactam antibiotics conversions , the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga . both the constructed plasmids psmlfpga and pkkfpga contained the pga gene , trc promoter and rrnb transcript terminator , differ in the replicon and antibotic marker , pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker . while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker
本文将粪产碱杆菌青霉素g酰化酶( afpga )基因构建表达载体pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc启动子、青霉素g酰化酶基因、 rrnb转录终止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷贝复制子；而psmlfpga含有四环素抗性基因和p15a中拷贝复制子。 |
- Similar Words：
- "青霉素b" English translation, "青霉素bt" English translation, "青霉素f" English translation, "青霉素f, 戊烯-2-青霉素" English translation, "青霉素f钠" English translation, "青霉素g,青霉素钠,苄青霉素钠" English translation, "青霉素g,苄青霉素,盘尼西林,配尼西林" English translation, "青霉素g氨乙酯" English translation, "青霉素g二苯甲胺盐" English translation, "青霉素g二乙氨基乙酯" English translation
|
|
|
