| 1. | Rules of indirect elisa technique for equine infectious anemia disease
马传染性贫血病间接elisa技术规程 |
| 2. | And the binding specificity of the scfv fusion protein with hbsag was confirmed by indirect elisa
间接elisa检测证实所表达的单链抗体具有与hbsag结合的特异性。 |
| 3. | Test 3 : detected activity of serum and immunoglobulin samples by indirect elisa test , rabbit antibody against foxes ' igg and hen ' s labeled with hrp igy was used in indirect elisa test
试验三：间接elisa检测的抗体活性。用过氧化物酶标记的兔抗狐igg和鸡igy抗体进行间接elisa测定获得的样品的活性。 |
| 4. | It was testified that the antibody can special immunological recognition the protein gst - cpti and cptl by indirect enzyme linked immunosorbent assay ( elisa ) . the coefficient of correlation is significant and the potency is more than 1 : 800
经间接elisa法检测，抗体能与gst - cpti 、 cpti蛋白特异性结合，其相关系数达到显著水平，效价1 800 。 |
| 5. | 2 . the putative epitopes that displayed on phages were identified by indirect elisa using swine antisera against prrsv and mouse antisera to recombinant structural protein of prrsv . seven putative epitopes could be recognized by antisera
利用prrsvbj - 4阳性血清和鼠源抗重组结构蛋白抗体，采用间接elisa方法对噬菌体展示的表位进行了鉴定。 |
| 6. | The activity of the purified product was confirmed by indirect elisa analysis and was further confirmed by indirect immunofluorescence and immunohistochemistry after they were added to the culture medium of hepatocarcinoma cells
在大肠杆菌blzi中实现了scfv融合蛋白的表达。经ni nta柱纯化获得纯化产物，经间接elisa分析确定所得产物具有与hbsag结合的特异性。 |
| 7. | Following the purification of the expressed protein , a elisa was developed to detect reference antisera of h7 , h5 and h9 subtypes . the result indicated that h7 antisera was positive , h5 and h9 antisera were negative
用电洗脱方法纯化菌体表达ha1蛋白后，建立了间接elisa方法，并对aivh7 、 h9 、 h5亚型血清进行检测，结果h7亚型血清呈阳性， h5 、 h9亚型血清呈阴性反应。 |
| 8. | Vaccine , and to monitor the antibodies level of unvaccinated ducks . the response curves were established when titers were varies along with the time , and the results showed that the indirect elisa could used to predict the efficiency of vaccination
应用建立的间接elisa方法，对40只用油佐剂灭活疫苗免疫后鸭子的抗体消长进行了测定，结果表明该方法适于作为疫苗免疫效果的评价。 |
| 9. | All the clones could react with swine antisera against prrsv and mouse antisera to prrsv , suggesting aa50 ~ aa55 domain on n protein might be the antigenic site recognized by mab ge3 . 4 . the gene fragment encoding the epitope recognized by mab ge3 was cloned into the expression vector pgex - 4t - 2
用prrsv阳性血清和鼠源抗prrsv抗体采用间接elisa进行鉴定，结果表明筛选到的噬菌体克隆可以与血清发生特异性反应，从而初步确定了单克隆抗体ge3所识别的抗原表位。 |
| 10. | The comparison of antigen - capture indirect eljsa ( aci - elisa ) experiments showed the corporation of ac9 and cf8 could doubly enhance reaction sensitivity than ac9 or cf8 alone . throught chessboard test found out the most suitable dilution of ac9 - cf8 and the csfv postive standar serum respectively is 1 : 400 and 1 : 200
用ac9和cf8单抗对csfv进行抗原捕获间接elisa试验( aci - elisa ) ，通过一元单抗和二元单抗cai - elisa试验的比较，结果表明ac9与cf8两种单抗协同作用可使elisa反应值提高1倍。 |
