| 1. | Metallothionein - 3 ; yeast two - hybrid system ; interaction ; dimerization
金属硫蛋白- 3酵母双杂交系统相互作用二聚体 |
| 2. | Yeast two hybrid system assay indicated that aes and tle1 interact by their homologous q domain
Dna的酵母双杂交载体，通过酵母双杂交系统分析证实aes与tlei分子通过氨基端的q结构域相互结合。 |
| 3. | Detection of interactions among metallothioneins by yeast two - hybrid system detection of interactions among metallothioneins by yeast two - hybrid system
酵母双杂交系统检测金属硫蛋白mt间的相互作用 |
| 4. | In y2h , rapgap from c . elegans was used as bait to screen c . elegans cdna library . after - lth screen , 63 colonies were checked
在酵母双杂交系统中，将ppc97 rpg ”与ppc86七dna文库共转化入酵母菌y190细胞，经过一lth筛选后，有63个菌落拟为阳性菌落。 |
| 5. | Metallothionein - 3 , previously named as growth inhibitory factor , is the unique member of the metallothionein family that demonstrates bioactivity in vitro . to test if mt - 3 dimerizes or polymerizes itself
为了验证其在细胞内是否形成二聚或多聚体，构建酵母双杂交系统用于检测。 |
| 6. | In order to study the upstream and downstream components in g protein and calmodulin signal pathways , a yeast two - hybrid system was used to screen interaction partners of these two proteins
本文利用酵母双杂交系统分别筛选了与这两种蛋白相互作用的蛋白质，以进一步研究g蛋白和cam信号转导途径中的其它上下游组分。 |
| 7. | The yeast two - hybrid system is an effective method for the study of protein - protein interaction in recent years . it is widely used in the study of life science because it provides a genetic means of identifying proteins that physically interact in vivo
酵母双杂交系统是近年来发展起来的一种研究蛋白质?蛋白质相互作用的有效方法，由于它检测的是一种体内的相互作用，自这一系统建立以来，就被广泛地应用于生命研究的各个领域。 |
| 8. | Even so , by truncating hbv pres gene , we finally obtained some useful " " bailors " , either nontoxic or self - activating , and used them to fish dna fragments of hbv pres interacting protein ( s ) from an ad vector constructed human embryonic cdna library
我们通过第回军巨大学硕士学位论文对pres基因分段截短的方法，获得了对酵母细胞即无毒性作用，又没有自激活作用的“诱饵” ，通过它在酵母双杂交系统中筛选构建于ad载体的人胎肝。 |
| 9. | We obtained two positive clones after screening . sequencing and blasting results showed that the two clones had the same sequences and encoded part of ked like protein in arabidopsis . the coding region was from n terminal amino acid 240 to c terminus
测序及序列比对结果表明二者序列相同，为ked样蛋白的部分编码序列，编码部分从n端第240位氨宋林夜:利用酵母双杂交系统筛选与g蛋白及cam相互作用的新型蛋白基酸至c末端，共207个氨基酸。 |
| 10. | Using yeast two - hybrid system honjo ' s group in kyoto university isolated a novel protein , kyot , which physically interacts with rbp - j . differential splicing gives rise to three transcripts of the kyot gene , kyotl , kyot2 and kyots . kyot2 , but not kyotl negatively regulates transcription by association with rbp - j
为进一步探讨rbp - j的功能，日本京都大学的honjo研究组利用酵母双杂交系统筛选到一个可以与rbp - j相互作用的新蛋白kyot ， kyot蛋白有三种不同的剪切体： kyot1 ， kyot2和kyot3 ，其中kyot2可以与rbp - j相互作用抑制其转录活性。 |
