English translation for "轻链可变区"

variable region of light chain
variable region of the light chain

Related Translations:

可变:  variable; changeable◇可变比 variable ratio; 可变成本 direct costs; variable costs; 可变电容 variable capacity; variable capacitance; 可变电容器 variable condenser; variable capacitor; variodenser; adjusta

轻:  Ⅰ形容词1.(重量小; 比重小) light 短语和例子身轻如燕 light as a swallow; 油比水轻。 oil is lighter than water. 这箱子轻得出人意外。 the suitcase is surprisingly light.2.(数量少; 程度浅) small in number, degree, etc.: 年纪很轻 be very

Example Sentences:

	1.	The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library . one of positive scfv clones , named pcsal , was selected with phage - elisa after panning and screening by bull sperm three times . scfv fragment , amplified from pcsa1 , was ligated to pmd18 - t vector for sequencing analysis 取阳性重组噬菌体抗体克隆株pcsa1 ， pcr扩增其scfv基因，筛选重组子进行序列测定，发现其序列符合小鼠抗体基因的一般特征，并且与几株抗磷酸胆碱的抗体重链和轻链可变区序列的同源性达80以上；推测pcsa1scfv针对的抗原是磷酸胆碱类物质。
	2.	Thus , immunologists have sought smaller molecules with the antigen - recognition capability of antibodies . the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable . it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues 二、日本血吸虫单克隆杭独特型抗体np30的单链狐（ scf ）的构建、表达及对balbic小鼠诱导保护性作用研究l 、日本血吸虫单克隆杭独特型抗体np0的单链抗体归cfv ）的构建、表达通过pcr方法体外扩增并经测序验证的重链、轻链可变区（ vh 、 vl ）基因先后重组入原核表达质粒ptha90相应的位点上，中间通过一连接肽（ gly在er ） 。
	3.	In the present study , the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis . total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies . after obtained using rpas system , vh and vl genes were used to assemble scfv gene fragment with a linker primer 应用重组噬菌体抗体库技术，从分泌小鼠抗牛精子sp18抗体的杂交瘤细胞系中分离总rna ，克隆抗体重链和轻链可变区基因，加入连接肽引物( linkerprimer )组装成单链抗体scfv ( singlechainfragmentvariable )基因并用rs引物进行扩增， sfi 、 not酶切，回收后与pcantab5e载体相连，转化e . colitg1宿主菌，构建单链抗体文库。
	4.	Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2 . construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr . the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e . co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii ) Mg _ 7重组噬菌体抗体库的构建及鉴定从培养的mg _ 7杂交瘤细胞中提取并分离mrna ，反转录成cdna ；利用pcr分别扩增mg _ 7单抗的重链及轻链可变区基因，并通过? dna连接子将二者连接起来形成mg _ 7单链抗体基因；将mg _ 7单链抗体基因插入pcantab5e ；将连接产物转化感受态tg1大肠杆菌，制备细菌形式的mg _ 7重组噬菌体抗体库；通过菌落计数和限制性酶切分析( ecor和hind )评估mg _ 7重组噬菌体抗体库的容量和重组率。

Similar Words：

"轻链标记物" English translation, "轻链病" English translation, "轻链沉淀病" English translation, "轻链蛋白尿" English translation, "轻链恒定区" English translation, "轻链球菌" English translation, "轻链肾病" English translation, "轻链顺序" English translation, "轻链稳定区" English translation, "轻链相关性淀粉样变性" English translation