| 1. | A method to construct 12 kb plasmid vector
重组质粒载体的方法 |
| 2. | By using ssh , a cdna subtractive library of aloe under salt stress had been done
以经过驱逐方cdna消减的第二次pcr产物与t a质粒载体连接，建成cdna消减文库。 |
| 3. | Result , we succeed in doing t - pa gene from fetal lung and construct a sort of eukaryon expression plasmid vector with pcdna3 . 1 ( + )
结果：成功地从胎儿肺组织中克隆了t - pa基因，并构建了以pcdna3 . 1 ( + )为载体的真核表达质粒载体。 |
| 4. | In order to construct plant artificial chromosome , plasmid vectors have been constructed to integrate necessary elements into both right and left yac arms
为了构建植物人工染色体，我们构建了可以和含有着丝粒序列的yac载体的左臂和右臂进行同源重组的质粒载体。 |
| 5. | Hydrophobicity analysis suggested that the protein was highly hydrophilic , especialy at the first 24 amino - acid , this region could be function as signal peptide
将此基因重组到表达质粒载体pgex - 4t - 1上，转化到大肠杆菌bl21 ( de3 )中，经iptg诱导后，可得到高效表达。 |
| 6. | Haake da , champion ci , martinich c , et al . molecular cloning and sequence analysis of the gene encoding ompl1 , a transmembrane outer membrane protein of pathogenic leptospira spp [ j ] . j bacteriol , 1993 , 175 ( 13 ) : 4225
晏菊芳,鲍朗,伍卫华,等.中国钩端螺旋体强毒株017膜蛋白基因的质粒载体构建及表达[ j ] .中华微生物学和免疫学杂志, 1999 , 99 ( 2 ) : 117 |
| 7. | 2 . ecori / bamhi digested pcr products were inserted into the corresponding restriction site in the expression plasmid pbv220 . construction of non - fusion expression plasmid pbv220 - endostatin
用ecori和bamhi酶切endostatincdna的pcr产物，将其插入到质粒载体pbv220中相应的限制性酶切位点，构建非融合质粒表达载体pbv220 - endostatin 。 |
| 8. | Furthermore , we discussed the influence of immune - sensibilization before induction to the activity of t - cell vaccine , compared the immune - sensibilization difference between hcv - adenovirus vectors and plasmid vectors
此外，我们还探讨了诱导前免疫致敏对t细胞疫苗活性的影响，并比较了hcv腺病毒载体和质粒载体在此免疫致敏功能方面的差异。 |
| 9. | Objective , clone tissue - type plasminogen activator ( t - pa ) gene and construct a new kind of recombinant vector containing human tissue - type plasminogon activator ( t - pa ) cnda neither cytotoxiaty nor actovating prot - oncogenes
目的：克隆组织纤溶酶原激活物( t - pa )基因并构建一种无细胞毒性、不激活原癌基因的真核表达的pcdna3 . 1 ( + ) / t - pa质粒载体。 |
| 10. | The recombinant expression plasmids prt - p450nor and pet - p450nor were constructed by inserting p450nor gene into the bamh i / hind iii site of the prokaryotic expression vector prset and pet28 , then they were transformed into e . coli bl21
本研究将已克隆的真菌细胞色素p450nor基因插入原核表达质粒载体prset和pet28的bamhi / hind位点，成功构建重组表达质粒prt - p450nor和pet - p450nor ，并转化到e . colibl21 。 |
