| 1. | The construction of cdna expression library from the tentacles of sagartia rosea
表达文库的构建和初步分析 |
| 2. | Basing on these backgrounds and motives , the cona expressing library of plasmodium falciparum from hainan was constructed
正是基于以上目的和研究背景，我们构建了恶性疟原虫海南株红内期cdna表达文库。 |
| 3. | 3 fifty candidates proteins possible interacting with the nthk1 protein were screened from the tobacco cdna library through the yeast two - hybrid system
3利用酵母双杂交技术从烟草的cdna表达文库中筛到50个可能与nthk1的相互作用的蛋白基因。 |
| 4. | Tanaka reported a novel method to isolate genes of drug - binding proteins directly from a cdna expression library by using the combination of drug and marker molecule
即利用标记分子与药物结合作为探针，直接从cdna表达文库筛选药物结合蛋白的基因并分离和体外表达，以便获取目的蛋白进行结构和功能研究。 |
| 5. | In the present study , a cdna expression library was constructed according to smart cdna library construction kit user manual from the adult amphioxus guts using pcdna3 eukaryotic expression vector
本文应用smart ~ ( tm ) cdnalibraryconstructionkit的方法，以成体文昌鱼肠为研究材料，构建了以真核表达载体pcdna3为基础的cdna表达文库。 |
| 6. | Then the recombinant dna was packaged in vitro . . identification of cdna library was followed , such as clone efficiency , recombinant efficiency , library integrality and the size of inserts
第二部分：以gt11为载体构建乳腺癌细胞t47d的cdna表达文库；对构建的文库进行克隆效率、重组效率、完整性以及插入片段的鉴定；并进行文库扩增。 |
| 7. | Functional study on these novel genes is an on - going task in our laboratory . amphioxus amphihmgb encoding a hign - mobility - group ( hmg ) protein was identified from the gut cdna library of the amphioxus b . belcheri tsingtauense
文昌鱼肠cdna表达文库的成功构建，为从分子生物学水平研究文昌鱼的进化与发育奠定了基础，也是分离新基因的重要来源。 |
| 8. | 5 out of approximately 120000 plaques , ten positive cdna clones were isolated from a fe - deficiency root cdna expression library of malus xiaojinensis by screening library for three times using tamre - bp cdna as a probe , then two representative clones were 5 " sequenced
5 、用小麦金属反应元件tamre - bpcdna作探针，筛选小金海棠缺铁根cdna表达文库，通过对约120000pfu的文库进行三轮筛选，共获得10个阳性克隆。 |
| 9. | The cdna expression library that contains no intron theoretically include all expressed genes and conserve resource genes permanently . lt can be used to find out and clone some genes which can express particular protein with modern molecular biological techniques , such as immunological screening , drug - prob screening , southern et al . lt is very important to study the life nature of plasmodium falciparum in molecular level . with the developments of these studies , the drug - resistant mechanism of the plasmodium falciparum and the genes of some specific medicine binding protein can be made well - known . at the same time , the researches will do good to explain the mechanism of some specific medicines in order to design and screen new anti - malaria drugs
建立cdna表达文库在一次永久保存基因资源的同时，可以利用功能筛选、免疫学筛选、药物探针筛选、 southern杂交和大规模序列测定等现代分子生物学技术寻找特异性活性蛋白基因，进而克隆和表达这些基因，对从核酸及蛋白质等分子水平研究疟原虫的生命活动规律，对揭示其抗药性分子机理，搞清某些特效药物结合蛋白的基因及此类药物的作用机制，对新型抗疟药物的合理设计及筛选都具有极其重要的现实意义。 |
| 10. | In order to clone new genes expressed during early embryonic development of trionyx sinensis , we constructed and characterized a cdna expression library from poly ( a + ) mrna isolated from 250 mg of cranial / kidney / gonad complex tissues of one - week - old embryos of trionyx sinesis using the smart ( switching mechanism at 5 " end of rna transcript ) cdna synthesis and ld - pcr amplification strategy
为了克隆到与胚胎发育有关的新基因，以孵化一星期的中华鳖( trto尸砂优sinensis )胚胎的头部、生殖晴混合组织为原始材料，采用smart但witching丛eehanism丛5 ’ endof旦nairanseript )和长距离pcr伍d一pcr )技术，构建t一个中华鳖cdna表达文库。 |
