English translation for "蓝白斑筛选"
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- blue-white selection
Related Translations:
白斑: hickie; leukasmus; vitiligo; floccosoids (如铁杉)◇白斑病 leucoplakia; leucoderma; leucodermia; melanodermia; piebald skin; vitiligo 宫颈白斑: leukoplakia of cervix 先天白斑: albinismcongenital leukodermia
- Example Sentences:
| 1. | The immunization effects of b95 can be disturbed by hi20 if the immunized ? interval less than 10 days with the normal dose
将此片段与pbluescriptsk载体连接，转化大肠杆菌tgi ，经蓝白斑筛选，鉴定阳性克隆后测序。 | | 2. | 713bp and 700bp specific fragments were amplified by pcr and ligated into pgem - t easy vector . it was identified by restriction endonuclease digest analysis , pcr and sequencing that this fragment contained the complete open reading frame ( orf ) of the hc and ha gene
扩增产物连接到pgem - teasy载体上，转化入大肠杆菌jm109中进行蓝白斑筛选后，用酶切、 pcr鉴定和测序的方法鉴定出重组阳性质粒( pgem - hc和pgem - ha ) 。 | | 3. | The 1 . 488kb dna fragment was sequenced and ligated to pbi121 vector and transformed into otsa deficient of e . coli strains ( ff4169 ) . otsa gene is in charge of trehalose - 6 - phosphate synthesis in e . coli . in growth curve experiment , the transformants that carried the 1 . 488kb dna fragment grew well in minimum medium , which contains 0 . 5mol / l nacl , while control strains could n ' t endure it
提取酿酒酵母的总dna ，以此为模板，采用pcr的方法从酿酒酵母中克隆出了1 . 488kb的海藻糖合成酶基因tps1片段，通过xba和sma双酶切，与同样经过xba和sma双酶切的puc118载体质粒连接，转入大肠杆菌dh5中，通过蓝白斑筛选重组子。 | | 4. | In order to construct the recombinant plasmid pcdna3 . 1 / ts87 , first , the ts87gene fragment was repcred using the redesigned primers to introduce re sites of hindld and bamh i , and kozak sequence . then the rebuilt ts87 fragment was cloned into t - vector and transformed into e . coli dh5 a
通过重新设计引物在ts87两端加上用于构建重组质粒的酶切位点hind和bamh和用于真核表达的kozak序列。将改造后的基因片段克隆入t - vector ，转化大肠杆菌dh5 ，通过蓝白斑筛选获得阳性克隆后测序鉴定。 | | 5. | The e2 gene was amplified by rt - pcr , then examined the fragment by electrophoresis . after purification and insertion into pucm - t vecter , the recombinant plasmid pbne2pi and pbne2pii were obtained . then they were transfected e . coli jm109 and screened positive clones by blue or white plaques . the recombinant plasmids were extracted and the inserted fragments were identificated by electrophoresis
通过rt - pcr扩增e _ 2基因，电泳测定扩增片段的大小，经纯化后，连接于pucm - t载体，获得重组质粒pbne2p 、 pbne2p ，转化e ? colijm109 ，经蓝白斑筛选，挑取阳性克隆，提取质粒，直接电泳鉴定和酶切鉴定。 | | 6. | In this paper , a field strain of infectious bronchitis virus was isolated from proventriculus tissue , morphological observation by electron - microscope and the biological characterizations of the virus were studied , pairs of specific primers are designed and synthesized in correspondence with them , according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes , the cdna of si gene , s2 gene , m gene . n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported
在此基础上，根据国内外已发表的ibv基因序列，分别设计特异性引物，应用不同引物进行反转录合成cdna ，分片段对ibv的主要结构基因进行pcr扩增，并分别将各个目的片段克隆到puc19载体上，在大肠杆菌dh5中实现目的基因的分子克隆，经蓝白斑筛选、限制性内切酶分析、 pcr鉴定，筛选出重组阳性质粒，并对各个目的基因片段进行序列测定，从而获得ibv主要结构基因全序列。 |
- Similar Words：
- "蓝巴勒避风塘" English translation, "蓝巴勒海峡" English translation, "蓝巴勒海峡大桥" English translation, "蓝白" English translation, "蓝白矮星" English translation, "蓝白方格布" English translation, "蓝白管土" English translation, "蓝白海豚" English translation, "蓝白红三部曲" English translation, "蓝白红三部曲之白" English translation
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