| 1. | The work for screening transgenic plant which expresses yc2 . 1 is in progress
转基因蓝猪耳植株构建工作已取得阶段性结果。 |
| 2. | 2 ~ 3 days for co - cultivation were found to be sufficient for this transformation
2 3天共培养有利于蓝猪耳的遗传转化， 10 30 |
| 3. | The effects of varying conditions on torenia transformation were investigated
不同菌株对蓝猪耳的遗传转化能力不同， r1000和a4明显高于r1205和r1601 。 |
| 4. | And portulaca grandiflora hook . . the ability of calli formation of different torenia explants is distinct
对于蓝猪耳的愈伤组织诱导而言，不同外植体类型之间存在很大的区别。 |
| 5. | We transferred the hetero - logous gene into toreniafournieri successfully . this method laid a foundation for study of double fertilization of angiosperm by transgenic tecnology
我们将外源基因成功地导入蓝猪耳，为利用转基因的手段研究被子植物受精和胚囊发育打下基础。 |
| 6. | Therefore , toreniafournieri is a very suitable model plant for studies of double fertilization in living material . we transferred the fusion gene ofgfp : mtn to toreniafournieri by leaf disc transformation mediated by agrobacterium and gain transgenic plant
我们将嵌合基因gfp : mtn通过叶盘法导入蓝猪耳，探索了蓝猪耳遗传转化的方法和转基因苗的再生条件，建立了成熟的蓝猪耳转化系统。 |
| 7. | In the experiment , we have explored optimal conditions for the transformation of torenia fournier , and transformated it with antisense acc synthase gene to get transformated torenia fournier plants with flower longevity extension and more tolerance to abiotic stress
本研究探索和完善了蓝猪耳遗传转化条件，并将反义acc合酶基因转化蓝猪耳，以期获得延长花寿命，和有较高抗性的转基因蓝猪耳植株。 |
| 8. | Before anthesis wall of egg apparatus is complete ; most part of egg wall at the chalazal end disappears at the day of anthesis ; after frtilization ( 20h after pollinating ) egg wall becomes intact . the results suggest that rebuilding of wall is one of the early cytological events during fertilization
结果表明，开花前蓝猪耳的卵细胞的胞壁是完整的；开花时胚囊已发育成熟，此时卵细胞在合点端部分区域是裸的；受精后(大约授粉后20小时)卵细胞的胞壁恢复完整。 |
| 9. | In this research , we constructed the expression vectors , which contain exogenous gene chalcone systhase ( chs ) antisense gene of gerbera hybrida and transformed it into agrobacterium lba4404 through freezing - melting . an efficient regeneration system and transformation system have been estadblished on leaf discs of torenia fournieri lind
以蓝猪耳为主要研究对象，建立了高效的蓝猪耳离体再生体系，并以蓝猪耳叶片为转化材料，用带有目的基因的农杆菌感染建立了蓝猪耳的遗传转化体系。 |
| 10. | ( 2 ) compared with wild - type torenia fournier , antisense acc synthase gene transformated plants of torenia fournieri had the following changes : the rate of ethylene biosynthesis dramatically decreased in the plants . cholrophyll content in the leaves is 1 . 15 times of that of the wild - type torenia fournier leaves . the transformed plants had more flowers
( 2 )反义acs转基因蓝猪耳与野生型的蓝猪耳相比较，乙烯的生成速率显著下降：叶片叶绿素的含量提高，是野生型的1 . 15倍；花开的更多，花的寿命增加一天；单个果实的种子的数量和重量均有所增加；叶片多胺的含量显著高于野生型的蓝猪耳；植株对氧化胁迫、酸胁迫、干旱胁迫的抗性均有增加。 |
