Through primary pcr . and finally the recombinant dna fragments were cloned into the phagemid pcantab 5e vector and introduced into 十五肽的接头连在一起，并克隆到噬菌质粒pcantab 5e中，电击转化大肠杆菌tg1 。
2.
Nucleic acid sequences of azoreduclase were searched and blasted in genbank . a pair of primers based on the conserved regions were designed . a specific fragment was amplified by pcr from the plasmid of rhodopsedomonas palustris and sequenced . the sequence contained a complete 471bp orf ( open reading frame ) 脱色实验证明沼泽红假单胞菌( rhodopsedomonaspalustris )对偶氮染料有较强的降解能力，我们通过genbank搜索，对所获得的所有偶氮还原酶基因在ncbi进行比对并设计引物，从沼泽红假单胞菌质粒中扩增获得了一条含471bp完整开放阅读框架的序列。
