| 1. | Observations with fluorescent staining of amitosis in nitellopsis obtuse
荧光染色观察钝节拟丽藻中无丝分裂的研究 |
| 2. | Those of micro - ball with different fluorescent dye can be used in biochemical analyses and biology molecules will react on the micro - ball
利用带有两种荧光染色剂的微球进行生物分析，生化反应将在悬浮于液体中的球基上进行。 |
| 3. | First we employed c . w . m2r , a fluorescent fabric brightener , to investigate changes of wall of female germ unit before and after fertilization
通过荧光染色技术，我们首先观察了自然受精过程中胚囊内雌性生殖单位细胞壁的变化。 |
| 4. | If an immunofluorescence stain with antibody to complement or immunoglobulin is performed , then one can see the brightly fluorescing band along the dermal epidermal junction that indicates immune complex deposits are present
如果针对补体的抗体或免疫球蛋白进行免疫荧光染色,我们就能在表皮与真皮的交界处看到一条明亮荧光带，表明有免疫复合物的存在 |
| 5. | In our report , we selected trehalose to substitute sucrose in the cryoprotectant . the process of vitrification is under the same preferable condition . the survival - rate of the recovered cells was tested by fda - pi and cd34 + cell - count
冻融后的细胞以fda - pi双荧光染色、流式细胞仪cd34 +计数等检测手段，与同样条件的60蔗糖保护剂处理的细胞相比较。 |
| 6. | 6 . oocytes were fixed for immunofluorescence . examination of cgs and microtubules were performed by fitc labeled lens culinaris agglutinin ( lca ) and and - fi - tubulin under confocal scanning laser microscopy ( cslm ) respectively
利用直接免疫荧光染色和共聚焦显微镜（ confocalscan muglasermicroscopy ， cslm ） ，研究各组体夕成熟卵的皮质颗粒和微管 |
| 7. | The cell microarrys were dyed with trypan blue , wrights , three colors fluorescence and papanicolaou strained . results leukocyte samples from 20 all patients showed predictably and distinctly different dot patterns from samples from 20 normal subjects
将杂交后的细胞芯片进行胎盘兰染色、瑞氏染色、 cd3 cys cd4 fitc cds rpe三色荧光染色、巴氏染色等并观察结果。 |
| 8. | Throughout the culture period , most cells within the colonies continued to be alkaline phosphata se positive and specific stage embryonic antigen ( ssea - 1 ) positive which has been used routinely to characterize embryonic stem and pgcs cells
经过4 7天培养， pgcs形成典型的鸟巢状集落。集落在传代过程中保持碱性磷酸酶活性，胚胎阶段性特异抗原1 ( ssea - 1 )免疫荧光染色显示阳性。 |
| 9. | The survival - rate of the recovered cells was tested by mtt and fda - pi . therefore , we got the preferable condition of vitrification , i . e . , the concentration of cryoprotectant is 60 % and the time of disposal is 15min . under this condition , the value of fda / pi is 46 . 43
冻融后的细胞经过mtt检测和fda - pi双荧光染色法检测，获得预处理过渡的优化条件，即60浓度的保护剂，预处理15分钟，此时的fda pi值为46 . 43 。 |
| 10. | Materials and methods the mouse , golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation , respectively , and ar was induced by progesterone after capacitation , then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method . the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf , then the fertilization rate was observed . the 1 - cell , 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin , then the rate of blastocysts was recorded
方法取小鼠精子10份、金黄地鼠精子6份、人新鲜精液标本10份及人冷冻精液标本9份，分别与不同浓度内毒素共孵育进行体外获能和孕酮诱导的顶体反应，应用金霉素和dna结合的荧光染料hoechest33258双重荧光染色法检测精子的获能率和顶体反应率；小鼠体外受精实验的精卵结合环节培养液中加入不同浓度的内毒素，观察受精情况并记录受精率；取小鼠1 -细胞胚胎、 2 -细胞胚胎和去卵透明带2 -细胞胚胎，与不同浓度内毒素共孵育进行体外培养，观察体外发育情况并记录囊胚率。 |
