Physiological and biochemical characteristics of development of heterosis in brassica crops 芸薹作物杂种优势形成的生理生化特征
2.
Genetic diversity analyses based on ssr markers of brassica vegetable germplasm distributed in shanghai 上海地区芸薹属蔬菜遗传多样性研究
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Diagnosis and genetic analysis of resistance to cauliflower mosaic virus in brassica crops which was transformed with camv gene vi 芸薹属蔬菜植株抗病性鉴定及其遗传分析
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Techniques for anther culture has been developed and widely used for most brassica species , but not for radish ( raphanus sativus l . ) 花药培养是获得单倍体的有效途径之一，已在十字花科芸薹属作物上取得巨大成功。
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Chinese cabbage - pak - choi ( brassica campestris l . ssp . chinensis makino ) is a typical allogamy plant and is of attractive heterosis . and , it is also one of the most popular leafy vegetables , especially in the south china 白菜( brassicacampestrisl . ssp . chinensismakino )属十字花科芸薹属芸薹种植物，在我国农业生产和人民生活中占有非常重要的地位。
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In this review , advances and problems on the study of agrobacterium - mediated genetic transformation of brassica , such as establishment of efficient plant regeneration system and infection method are represented 从高频再生体系的建立、农杆菌的侵染方法、导入外源基因等方面综述了芸薹属作物的遗传转化研究进展，并对研究中存在的再生成苗、转化率低和筛选方法等问题进行了讨论。
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Abstract : in recent years certain progess in brassica signaling was reviewed about some self - compatibility - related genes such as srk , slg , scr , arc1 , thl1 and thl2 . meanwhile , molecular mechanism in brassica self - compatibility signaling was reviewed , including its action models 摘要综述了近年来在芸薹属植物自交不亲和信号转导途径中相关基因的研究进展，同时对其自交不亲和信号转导途径中的分子机制作一阐述。
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In sporophytically self - incompatible brassica , three s - linked genes , the pollen ligand scr , stigma receptor slg and srk , have been identified . in addition , four genes ( arc1 , thl1 , thl2 and mod ) that do not link to s locus have also been found , they are regarded as downstream protein factors of self - incompatibility ( si ) response , however , their biological function is not clear 在孢子体型自交不亲和的芸薹属中，已经鉴定出3个主要与s位点紧密连锁的基因，花粉中的配体scr和柱头中的受体slg与srk ：另外鉴定出4个不与s位点连锁的基因arc1 、 thl1 、 thl2和mod ，它们被认为是si反应的下游蛋白质因子，但是对它们的生物学功能了解的还比较少。
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Arc1 , thl1 and thl2 , the substrate protein genes of s receptor kinase , were cloned through a series of methods of molecular biology such as pcr , rt - pcr , dna cloning and sequencing . the resultings sequences were highly analysed by using the related biosoftwares on internet , providing new insights in the field of the molecular mechanism of self - incompatibility in plants . the major results are as followings : 1 本文通过pcr和rt - pcr等一系列分子生物学方法克隆了芸薹属植物中的甘蓝和油菜自交不亲和信号传导过程中srk底物蛋白基因arc1 、 thl1和thl2 ，并使用各种相关生物信息学软件对srk底物蛋白基因序列进行了分析，然后在internet网上利用在线软件对蛋白质的结构和功能进行了预测和探讨，以期为芸薹属植物自交不亲和性的分子机理的研究提供新的内容。
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Primers were designed according to the sequences of atcal , bocal , bobcal and boical gene , and pcr amplifications were performed with the genomic dna of brussel sprouts , kale and kohlrabi respectively , pcr fragments of about 1 . 6kb were obtained and designated correspondingly as bogcal5 " , boacal5 " and boccal5 " 根据拟南芥atcal 、结球甘蓝bocal 、花椰菜bobcal和青花菜boical基因设计引物，对芸薹属植物抱子甘蓝、羽衣甘蓝和球茎甘蓝的基因组dna进行pcr扩增，均得到一段约1 . 6kb大小的pcr产物，分别命名为bogcal5 ’ 、 boacal5 ’和boccal5 ’ 。
