| 1. | 1 shape of core plate
1胚体基本形状 |
| 2. | The embryonic shield appeared in 15h , and the fertilized egg entered the middle gastrula stage
15小时出现胚盾， 15小时55分，胚体下包一半。 |
| 3. | ( 2 ) the ratio of nucleus to cytoplasm about fibroblast increased gradually with culturing time in the same generation
( 2 )体外培养的原代小鼠胚体成纤维细胞随培养时间的增加，核质比逐渐增加。 |
| 4. | Embryo almost encircled the yolk - sac in 38h40min . the larva broke out of the egg in 39h50min , at that time , the number of the oil globules decreased greatly
38小时40分胚体绕卵黄囊约一周， 39小时50分仔鱼孵出，仔鱼孵出时卵黄囊内的油球已大大减少。 |
| 5. | Under suspension culture without cytokines , the putative eg clonies could be induced to form simple embryoid bodies . after long - time culture , these bodies began to differentiated into various cell types
将消化分散的类eg细胞在无分化抑制因子的类eg培养基中重悬培养， 3 4天后，可见部分细胞团形成简单的胚体。 |
| 6. | Eg cells of the 2th and 4th passage were akp ( alkaline phosphate ) positive . when cultured on degenerated feeder layers or in suspension , eg c ells formed embryoid bodies ( ebs ) in vitro
当eg细胞脱离饲养层悬浮培养，或在衰老的饲养层上延迟培养时，发现eg细胞或单个存在，或聚集成团，形成类似于早期胚胎的囊状胚体结构 |
| 7. | Spontaneous differentiation of es cell d3 line in vitro es cell d3 line via suspension culture can congregate small mass within 24 h , and large numbers of aggregates called embryoid bodies ( ebs ) gradually formed after 2 to 3 days of culture
5 . es一d3细胞系的自主分化es一d3细胞悬浮培养， 24h后可聚集成小的细胞团，第2 ~ 3d时形成大量的类胚体( ebs ) 。 |
| 8. | ( 4 ) the myocardium cell group on embryonic body fibroblast feeder was cultured successfully . the myocardium tissue grew gradually , its contraction changed with temperature and morphology changed with time , and not all myocardium tissue could contract
( 4 )小鼠胚体成纤维细胞可以与心肌细胞团共培养，并出现自律性收缩，细胞团逐渐增长，其收缩情况随温度变化而变化，随时间变化其形态学发生变化，而且并非所有心肌细胞团都能收缩。 |
| 9. | There is no different between the fibroblast feeder of mouse and the fibroblast feeder of goat according to blastoysts attached and icm proliferation . es - like cell develop embryoid body - like structure in suspension culture in vitro . es - like cells of goat differentiate into various kinds cell , egg : fibroblast cell , neurolike cell
Es细胞在体外悬浮培养，形成类胚体，山羊类es细胞在无饲养层铺有明胶的皿底上培养，可分化为多种类型细胞，如上皮细胞、成纤维细胞、神经细胞等。 |
| 10. | The ebs of different days gained by means of hanging , suspending and suspending following hanging were processed by all - trans retinoic acid ( ra ) for 4 days . during this period , we observed the differentiation process and compared the differentiation ratio of es cells processed by means of hanging , suspending for several days . based on this study , we induced the ebs by ra of different concentration in order to study the effect of ra on the differentiation of es cells into neuron - like cells
首先从胚体( ebs )本身探讨es细胞内部调控诱导分化的机制，用悬浮培养、悬滴培养、悬浮转悬滴培养的方法培养出不同时间的ebs ，再加以ra处理，观察比较ebs对es细胞神经分化的影响；在此基础上，用不同浓度的ra处理ebs ，分析诱导剂ra对es细胞神经分化的影响。 |
