| 1. | Culture of rabbit osteoblasts digested by collagenase in dmem containing fetal bovine serum
含胎牛血清胶原酶消化法培养兔成骨细胞 |
| 2. | The culture results of the porcine ear skin fibroblasts was good by using dmem / f12 containing 20 % fetal bovine serum
在培养条件方面选取dmem f12培养液+ 20胎牛血清培养猪耳皮肤成纤维细胞，效果较好。 |
| 3. | The numhers of day - 4 ebs per milliliter culture media are 44 . 83 1 . 22 ( n = 6 ) and 43 . 17 1 . 05 ( n = 6 ) respectively when cultured by hyclone fbs and homemade green season fbs
在含进口hyclone胎牛血清或国产四季青胎牛血清的培养液中培养， es d3细胞形成的4debs数量分另为44 |
| 4. | Pbmc were cultured in complete medium and supplemented with 1000u / ml rhgm - csf and 500u / ml rhil - 4 , modc were collected on day 9 . tumor cells were cultued with 10 % complete medium
肿瘤细胞的培养将mgc803 、 ls174t 、 ec109细胞分别培养于含10胎牛血清青霉素100n分ml ，链霉素100n分ml的rpmi |
| 5. | Results isolation , culture and purification of the mmscs typically , mmscs were isolated from bone marrow by their adherence to plastic and grew as fibroblastic cells that developed into visible symmetric colonies
显色15 30分钟。结果1 mmscs分离、培养及纯化用含10胎牛血清的dmem ? lg培养基在玻璃培养瓶中培养骨髓单个核细胞。 |
| 6. | Methods rabbit conjunctival epithelial tissues were explanted in serum - free system the morphology , gowth , proliferation and differentiation of outgrow cells were compared with those in serum - containing medium
方法分别用含胎牛血清培养液和不含血清培养液进行组织块培养，观察两种培养方法在细胞形态、细胞生长情况和增殖及细胞分化方面的差异。 |
| 7. | Methods isolation , , culture and purification of mouse mscs mmscs were isolated from the femurs of adult mice four weeks old and propagated in vitro . mmscs were originally cultured in dmem - lg with 10 % fbs , 100u / ml penicillion , 100mg / ml streptomycin
在细胞沉淀中加人含10胎牛血清的dmem lg培养基，计数细胞数，调整细胞密度，按3x10vinl接种于25cm ‘的玻璃培养瓶中。 |
| 8. | We can see a lot of new pictures of induced neuron - like cells , include neuron - like cells and glial - like cells , with the apparent nerve cells . material and methods rat bone marrow cell were collected , after sacrifice of a 3 months old wistar rat , from femurs and tibias by flushing the shaft with culture media ( dmem - 10 % fetal bovine serum ) using a syringe of 5ml
无菌条件下取股骨和胚骨的骨髓b人snd含10胎牛血清的dmem培养液1000rpm离心10分钟，吹打b人两个培养瓶，置人37 j coh饱和湿度条件下培养，隔日更换培养液，当细胞达到70融合时，传代。 |
| 9. | But in experiment ii , the obtained result of measurement and examination is not better than control group 。 conclusion : mixed animo acids can be safely applyed to vessel cryopreservation instead of calf serum 。 the effect of the groups of the rapid freezing method is not better than the groups of the rate - controled slow freezing
而实验ii的血管其内皮结构不清晰，内皮细胞超微结构变化较大。结论：复方氨基酸可代替胎牛血清用于血管的深低温保存.速冻降温方式对血管深低温保存效果不及慢冻降温方式。 |
| 10. | Method : 120 rabbit vessels were randomly divided into three groups : the vessel of control group in cryopreservative medium containing calf serum were subjeted to rate - controled slow freezing ; those of experiment group i in cryopreservative medium containing mixed animo acids were frozen by the same method as in control group ; and those of experiment group ii in the same cryopreserative medium as in control group were subjected to rapid freezing
材料和方法：取中国纯种家兔股动脉120段，随机分为3组：对照组用含胎牛血清的保护液处理，以慢冻方式降温；实验组1用含复方氨基酸的保护液处理，以慢冻方式降温；实验组ii用含胎牛血清的保护液处理，用速冻方式降温。 |
