| 1. | Frozen fresh specimens are used in page and pure ethanol humid preparations are used in cyt b gene 433bp sequence analysis
细胞色素b基因序列研究采用无水乙醇浸制标本。对扩增效果好的产物进行提纯和测序。 |
| 2. | The mitochondrial dma cytochrome b gene was sequenced from 32 siluriforms which are belonging to 22 genera and 10 families
本研究通过pcr技术获得了鲇形目10科22属32种鱼类线粒体dna细胞色素b基因1138bp全序列。 |
| 3. | Molecular systematics of partial species belonging to 5 subfamilies of miridae are investigated in this study by using esterase isozyme ( est ) and cytochrome b ( cyt b ) gene in mitochondrial dna as molecular marks
运用酯酶同工酶和线粒体dna中编码细胞色素b蛋白质的基因cytb作为分子标记，对盲蝽科miridae的5个亚科的部分种类进行了分子系统学研究。 |
| 4. | 3 . the sequence variation of mitochondria ! cytochrome b gene and phylogenetic relationships of anseriformes were studied based on the complete cytochrome b gene sequences of 19 species combined with 4 species got from genbank
3 、研究测定雁形目鸟类19f mtdna细胞色素b基因全序列，结合genbank中的4个物种，分析了雁形目鸟类细胞色素b基因的序列变异及其分子系统发生。 |
| 5. | In order to develop an accurate and efficient method for the identification of tiger dna , mtdna ctyb sequences of 5 tiger subspecies and 6 other feline species and 6 cervid species were downloaded from genbank , and compared by wdnasis ( v2 . 5 ) software
为了建立对虎dna进行特异性检测的有效、精确的方法，本研究中，从genbank下载虎及其他6种猫科动物和6种鹿科动物的mtdna细胞色素b基因序列，并用wdnasis ( v2 . 5 )软件进行了种间序列比较。 |
| 6. | Among avian orders , the anseriformes ( screamers and waterfowls ) are a morphologically and biologically diverse group containing ca . 150 species distributed worldwide except antarctica . this paper will review the phylogeny of anseriformes firstly and then discuss the molecular phylogeny of anseriformes based on the genetic variation of mitochondrial 12s rrna gene and cytochrome b gene respectively
本文首先对雁形目鸟类的系统发生研究进行了综述，然后分别基于雁形目鸟类线粒体dna12srrna基因和细胞色素b基因全序列的遗传变异分析，探讨了雁形目鸟类分子系统发生。 |
| 7. | Analysis of the sequence variation of cytochrome b gene indicated that there is no evidence of insertions or deletions , i . e . , they are all of identical length of 1143 bp in all the sequences of cytochrome b gene . further , the sequences can be fully translated into amino acid using chicken mitochondrial codon without nonsense mutations or intervening stop codons . the 1143 bp cytochrome b alignment contained 416 variable sites , of which 306 were parsimony informative sites with the strongest variable in third codon positions and less variable in first and second codon positions
细胞色素b基因序列变异分析表明： 1 ）雁形目鸟类细胞色素b基因全序列长度一致，无插入和缺失：对照鸡线粒体密码子系统全序列能全部翻译成氨基酸序列，无无义突变，全序列内部无终止密码子； 2 ）序列比对后1143加，含416个核着酸变异位点， 306个简约信息位点，其中处于密码子第三位的变异最大，第一位和第二位碱基的变异相对较小。 |
| 8. | Sequence variation analysis exhibits high transition to transversion substitution ratios , and transitions and transversions are unsaturated in the sequence of cytochorme b gene . the base composition of the cytochrome b sequences was highly biased . using a structural model of the cytochrome b protein as a template , we divided cyt b sequence data for species within anserifomes into three functional domains
3 ）序列具有较高的转换颠换比，基因序列中的转换与颠换都未饱和，序列中的碱基组成高度偏倚： 4 ）按照细胞色素为蛋白结构模型，细胞色素b基因全序列可分为三个功能区，碱基替换饱和状况与不同功能区相关联。 |
| 9. | All these characters were parsimoniously analyzed using paup4 . 0 . mitochondrial dna cytochrome b gene was adopted to discuss the sequences variations among the populations of sinibrama species and used to reconstruct the phylogenetic relationships between the genus sinibrama and some related cultrinae genera . on the basis of the analytical result , the hypothesized relationship of cultrinae by previous authors were testified
运用线粒体dna细胞色素b基因序列对华鳊属及其相关类群进行了系统发育重建，以探讨华鳊属鱼类不同地理居群的cytb序列变异及与其他相关类群的系统发育关系，并验证前人的一些研究结果。 |
