| 1. | The transient expression of mgfp4 in rice calli 4在水稻愈伤组织中的瞬时表达 |
| 2. | Transient expression of fibrillin promoter in protoplasts of arabidopsis thaliana and brassica napus 操纵子在甘蓝型油菜和拟南芥中的瞬时表达 |
| 3. | Acetosyringone ( as ) can increase the gus gene transient expression when it was added in the suspending medium 在菌液中添加as可以提高gus基因的瞬时表达。 |
| 4. | This result showed that gus was transiently expressed in the foliage , and they were of laticifer - expression 因此认为gus基因在叶片成功的实现瞬时表达,而且具有乳管表达活性。 |
| 5. | Pre - cultured 2 days and co - cultured 6 days can also enhance the efficienty of transformation of torenia fournieri lind 预培养2d后共培养6d ,可以明显增加gus基因的瞬时表达和不定芽的形成。 |
| 6. | Rt - pcr showed that the gfp transcripts from wild type nicotiana benthamiana inoculated with the tnos deletion vector lacked a normal poly ( a ) tail 接种野生型本生烟后的rt - pcr结果表明, gfp - atnos载体瞬时表达所产生的gfprna可能没有poly ( a )尾序。 |
| 7. | The ha 122 protein , when fused to gfp was observed in the nuclei of h . armigera cells , but only in conjunction with wild type hasnpv infection 将ha122与绿色荧光蛋白( gfp )基因融合,在昆虫细胞中瞬时表达。当有病毒感染同时发生时, gfp信号定位在核内。 |
| 8. | To identify its laticifer - expression character , two transient - expression vectors ( pirl , pbir2 ) were constructed . in the transient - expression vector , ref promoter sequence replaced camv35s promoter of pbi121 为验证其乳管表达活性,通过取代pbi121中间表达载体上的camv35s启动子,构建了两个含gus基因的瞬时表达载体pbir1 、 pbir2 。 |
| 9. | We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene , 2 . 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3 . 0 我们直接将含有4个内含子和自身信号肽的2 . 4kb全长人生长激素基因直接克隆至真核表达载体pcdna3 . 0 ,然后利用脂质体转染家蚕bmn细胞,瞬时表达hgh 。 |
| 10. | In the first part , the focus is to find the receptor molecules directly by screening two cdna libraries with a recombinant construct prpap or as an alternative , to find an enriched area in the embryo brains and construct libraries from this brain region and perform the expression cloning as above 方法: ( )以融合蛋白prpap通过瞬时表达克隆法筛选两个cdna文库,或者通过与胚胎脑的结合实验筛选有丰富结合蛋白的脑区,以图构建cdna文库并进行表达克隆的筛选。 |