| 1. | The sequence of hsei was deposited in the genbank
基因序列输入genbank中，登录号为af300796 。 |
| 2. | Results : a fragment of 896bp was got by rt - pcr , which contains the coding sequence of sh2 a gene
结果rt pcr扩增得到sh基因编码序列， genbank登录号为ay190323 ；连接到pcdna3 |
| 3. | Renature the purified target fusion protein through multistep dialysis . then unwater the frozen protein in order to store
本序列作为自中国人克隆的hpd一3被genbank登录，登录号为af516673 。 |
| 4. | Now , the structure gene sequence and 3 ' - end gene sequence have been logged on genbank . the accession number is ay044918
目前，此过敏蛋白tb22kda的结构基因与3 ’端基因序列已在genbank登录，登录号为ay044918 。 |
| 5. | Two partially overlapping cdna fragments which were cloned from o . violaceus using the rt - pcr , 3 ' race ( rapid amplification of cdna ends ) and 5 ' race technique was assembled a 1758bp full cdna sequence of epsp synthase ( accession number in genbank : af440389 )
采用rt pcr技术与3 ’ race和5 ’ race的结合，以诸葛菜的总rna为反转录的模板，克隆、拼结出了诸葛菜的epsp合成酶的全长。 dna序列（该段cdan的genbank的登录号为： af440389 ） 。 |
| 6. | Detailly , it was ( l ) to isolate and construct high efficiency expression vector of rice starch branching enzyme gene sbe2b and ( 2 ) to establish a high - effecient rice transformation system . total rna was extracted from maize endosperm 15dap ( days after pollination ) . and cdna of sbe2b was obtained through rt - pcr . sequencing result showed that the full length cdna was 2 . 4kbp , coding for 800 amino acids , with estimated mw 93kd
本研究从授粉15天左右的玉米胚乳中提取了总rna ，采用rt - pcr方法，克隆了玉米胚乳的淀粉分支酶基因sbe2b ，测序结果表明，玉米胚乳sbe2b的cdna全长为2 . 4kb ，编码800个氨基酸，推测的氨基酸的分子量为93000d ，该序列与genbank中登录号为af072725的玉米sbe2b的cdna序列有98的同源性，与水稻、大麦、小麦等禾本科植物的有关淀粉分支酶的dna序列都有极高的同源性。 |
| 7. | The full orp encodes a 487 - amino acid protein with a calculated molecule weight of 53 . 484 kda and an isoelectric point of 6 . 75 . at the primary sequence level , it shared high homology with clr , so was named clr - like serine protease , clsp . the clsp sequence has been submitted to genbank / embl ( genbank accession number af178985 )
该蛋白在核酸和氨基酸水平上与人补体组分cl :表现高度同源，故将其命名为补体cl :样丝氨酸蛋白酶(旦lr一like旦erineprotease ， clsp ) ，该基因已经在ge心ank登录(登录号为af17s985 ) 。 |
| 8. | These two subspecies are away from more than about 1 200 kilometers each other . the genomic dna of microtus fortis calamorum and microtus fortis fortis were extracted and amplified by pcr according to the specific genomic dna sequence of microtus fortis reported previously ( accession number in genbank : af277394 )
根据东方田鼠基因组dna序列片段( genbank登录号： af277394 ) ，用pcr技术扩增东方田鼠长江亚种( microtusfortiscalamorum )和宁夏指名亚种( microtusfortisfortis )基因组dna ，得到一670bp左右的特异扩增片段。 |
| 9. | Part 1 : identification of a novel gene , tsarg2 , and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell , and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis . to rapidly attain human novel gene full - length cdna sequence , the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe , which was significantly changed in cryptorchidism and represents a novel gene . furthermore , a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line
本研究分为三个部分，其主要实验方法及实验结果如下：第一章tsarg2基因的克隆与序列分析从已获得的在隐睾和正常睾丸对照中表达量有明显差异的est片段( be644542 )入手，设计了基因特异性引物和载体特异性引物进行巢式pcr扩增，结合人类基因组草图搜索法，从睾丸cdna文库中快速分离出人类睾丸凋亡相关基因的5末端而获得全长cdna ， genbank登录号为ay040204 ，同时应用生物信息学的方法克隆了该基因在小鼠中的同源基因， genbank登录号为af395083 。 |
| 10. | A pair of primers was designed based on the conserved regions of other higher plants " epsp synthase through homology alignments . two dna fragments were first cloned from o . violaceus by performing prc which used o . violaceus genome as the template . one has 798 nucleotides and the other 1157 nucleotides , but they can encode the same amino acid sequence and have same extrons according to the gt - ag rule of characteristic sequence of enkaryoutic intron
根据同源比较其它高等植物中epsp合成酶基因，找出该基因的保守序列并设计一对寡核苷酸引物，以诸葛菜的总dna为模板进行pcr反应，克隆出了两个epsps基因的片段，其中一条长为797bp ，另一条1157bp ，它们在genbank的登录号为： af440390 、 af440391 。 |
