2 mtt assay was used to examine od value of terasaki wells . od value was measured once a day for 8 days and mapped growing curve of cells to determine the activation of proliferation in primary cells 2采用mtt比色法测定细胞的光密度值( 0d值) ，每24h测定1次，从细胞接种到神经球形成连续测定sd ，绘制细胞增殖活性曲线。
