| 1. | Synthesis , crystal structure and properties of co 2 teta
配合物的化学核酸酶活性研究 |
| 2. | Expression and nuclease activity analysis of staphylococcus nuclease in e . coli
金黄色葡萄球菌核酸酶在大肠杆菌中的表达及其活性分析 |
| 3. | Milk and milk - based products - detection of thermonuclease produced by coagulase - positive staphylococci
乳和以乳为基料的制品.凝固酶-阳性葡萄球菌的耐热核酸酶检测 |
| 4. | These studies are helpful for elucidating the mechanisms of dna recognition and repair , and the design of novel synthetic nucleases
此项研究会对阐明生物体内dna的识别修复机理、合理设计新的人工核酸酶提供理论指导。 |
| 5. | The glycoprotein eo of classical swine fever virus ( csfv ) , besides being an envelope protein , possesses knase activity , which is pertinent to viral persistent infection in the host
猪瘟病毒( classicalswinefevervirus , csfv ) eo糖蛋白既是包膜蛋白，又是一种核酸酶，其活性对病毒在宿主体内的持续感染有直接关系。 |
| 6. | The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size . computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70 . 3 % that would encode a protein of 552 amino acids ( aa ) . the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp
对phz1754进行外切核酸酶( exonuclease ， exo )顺序缺失，获得单向长度渐减重叠的系列突变体，核苷酸序列测定显示出该ecor - sal片段的精确大小为2299bps ， frameplot程序分析揭示出该区域一个完整的开放阅读框( orf )的存在，其大小为1656bps ， g + c含量为70 . 3 ，编码552个氨基酸，利用blastsearch程序将orf的核苷酸序列及推导的氨基酸序列与因特网上基因及蛋白质数据库进行综合比较，发现无论在核苷酸水平还是在蛋白水平上，该orf均与胆固醇氧化酶表现出同源性，而且与链霉菌胆固醇氧化酶同源性最高，说明该orf编码胆固醇氧化酶基因。 |
| 7. | We designed a suitable probe in the experiment and used ribonuclease protection assay technology to detect the expression of pdipl gene in mouse liver at different time points after partial hepatectomy . we found that the expression of pdipl gene was increased . it reached the highest level after 12 hours and then decreased
我们设计合适的探针，采用核酸酶保护反应等技术测定了pdip1 -基因在肝脏部分切除后不同时间在小鼠肝脏中的表达，发现pdip1 -基因在肝脏部分切除后表达增强， 12小时表达最强，以后表达减弱。 |
| 8. | The results showed mn and ni complexes possibly bind to dna by the mode of interaction , whereas zn complex possibly bind to dna by the modes of interaction and electrostatic binding . 5 . in addition , we conjugated cleavage system with recognize system and analyzed joint products by hplc , which provide experimental basic for design of dual effects cleavage
此外，本文还选用咖啡酸纯品来突破切割体系与识别体系(用氨基臂修饰的寡聚脱氧核苷酸)的连接，并用高效液相色谱法分析其偶联产物，为今后设计并合成一种具有特异识别和高效切割双重功能的人工核酸酶提供了实验基础。 |
