Recombination gene was cut - down and introduced into the nuclei of oocytes or the cytoplasm of goldfish at one - cell stage via microinjection . the results as follows : ( 1 ) fluorescence was observed from embryo under suitable uv light after microinjection 36 hours . the fluorescent ratio of gastrula embryo period was up to 25 % 采用显微注射法将这种重组基因转化1细胞期的金鱼受精卵，实验结果如下： ( 1 )显微注射后，根据胚胎发育分期，胚胎在显微注射后36小时开始能在紫外灯下观察到荧光，原肠期发荧光的胚胎比例为25 ，后期发育荧光率逐渐下降，肌肉效应期后又相对稳定。
