| 1. | The cotyledons of the mature embryo are either green, yellow or chalky yellow .
成熟胚的子叶是绿色，黄色或浅黄色。 |
| 2. | In vitro culture of mature embryos of ginkgo biloba l
银杏成熟胚的离体培养研究 |
| 3. | The somaclonal variation of regeneration plant derived from mature embryo of perennial ryegrass ( lolium perenne l . were analyzed with rapd makers
摘要以多年生黑麦草成熟胚诱导获得的再生植株为材料，利用rapd标记方法对其体细胞变异进行了研究。 |
| 4. | When mature embryo was used as explant , as for above three lines of wheat , the optimize concentration of 2 , 4 - d for inducting calli and embryonic calli is 4 . 0 mg / l
35 ，而安农92484只能达到16 sl 。在所试的品种中对于成熟胚在2 ， 4d质量浓度为4 |
| 5. | Thus help the seedlings survive better in the flooding and saline conditions . the density of mature hypocotyls of b . gymnorrhiza was close to or lower than seawater , thus the propagules can float and be dispersed by tide
并通过主动的渗透调节，控制各种渗透调节剂和水分的输入，使得成熟胚轴的渗透势达到比生境中略低的一个比较合适的水平。 |
| 6. | The embryogenic calli induced from the mature embryos of forty - five genotypes of sweet corn varieties were treated as materials to analyze the relationship between plant regeneration and callus induction rate , callus proliferation and bud points
摘要以45种基因型甜玉米的成熟胚为外植体材料，分析出愈率、愈伤组织增殖量、芽点数与植株再生的关系。 |
| 7. | But cluster buds can not be obtained from the callus of stems and embryo . it has likely relate to the inner structure of callus . lt is very difficult to get embryoid from mature embryo and its inducing rate is very low , and only 11 . 5 % in the media containing n6 + ba 1 . 5mg / l + naa 0 . 5mg / l + sucrose 30g / l + agar 8g / l + ac 2g / l which is the best media for gingo tissue culture
而茎段和胚诱导的愈伤组织基本上没有丛生芽发生，这可能和不同愈伤组织的内部结构有关。成熟胚的胚状体诱导率较低，诱导相当困难，在n6 bai |
| 8. | Studies on transformation of indica rice with bt - toxin gene mediated by agrobacterium tumefaciens precultured immature embryo and callus derived from young panicle , immature embryo and mature embryo were used as acceptor for genetic transformation mediated by agrobacterium tumefaciens , the transformation rate of the above acceptor was investigated respectively . the results showed that immature embryo after precultured for 4 ~ 6d was the best . in respect to the concentration of agrobacterium tumefaciens when calli were cotransformated in medium yeb , to agrobacterium tumefaciens eha 105 , od value of 0 . 8 was the best
采用农杆菌介导法将bt毒蛋白基因导入水稻同样以上述两种籼稻为主要研究材料，比较了分别以预培养的幼胚和幼穗、幼胚、成熟胚来源的愈伤组织作为转化受体的愈伤组织转化频率，结果表明预培养4 6天的幼胚最适宜作为农杆菌介导转化的受体；其次是来源于幼胚和成熟胚的生长状态良好的胚性愈伤组织。 |
| 9. | The propagules of b . gymnorrhiza and a . corniculatum undergo some growth before becoming detached from the parent plant . they accumulated large amounts of ions during the development while the level of ion concentrations are consistently lower in their embryos compared to leaves of parent plant . before detachment , the growth of hypocotyls were reduced , the water content declined , while the osmotic potential was approaching to or more negative than that of the solution on which their parent plant vegetating
另一方面维持较大比例的有机渗透调节剂，控制细胞液中盐离子的水平以减少代谢毒害；木榄成熟胚轴的密度接近或低于生境中海水的密度，适于随水漂浮和传播；胚轴内离子浓度呈下降趋势，但最终以达到和母树生境中的离子浓度水平相一致为目标。 |
| 10. | Two aspects of research were performed on two varieties of indica rice ( oryza sativa l . ) pei ' ai64s ( pa64s ) and 9311 which were employed extensively as outstanding selectable parents of current super hybrid in this article . the main results were as follows : optimization of regeneration system suitable for genetic transformation of predominant indica rice several factors affecting indica rice pei ' ai64s and 9311callus induction , subculture , bud differentiation and plant regeneration were investigated with mature embryos as explants
本文以当前培育超级杂交稻正在广泛使用的两个优势亲本培矮64s和9311作为主要研究对象，对影响其成熟胚来源的愈伤组织诱导、分化和植株再生条件进行了优化；对影响农杆菌转化上述籼稻的条件进行了探索和优化；建立了高频的离体再生系统和比较高效的遗传转化方法。 |
