| 1. | To obtain full - length lzp3 cdna , a pair of primers was designed according to the assembled lzp3 cdna sequence
根据已拼接出的lzp3全长cdna序列设计基因特异性引物，利用rt - pcr方法扩增lzp3全长cdna 。 |
| 2. | According to the nucleotide sequence of selected antigen epitope , pcr primers supplemented with the ecor i and sal i sites were designed
根据选出的抗原表位区的核酸序列设计pcr引物，并于引物末端添加ecor和sa11酶切位点。 |
| 3. | Base on the encoding sequence of ast7 and gkr ( by which asts were concatenated ) , two primers were synthesized and constructed them in a proved pcr method
根据grb - ast _ 7和gkr ( ast神经肽之间的连接序列)的编码序列设计合成两个引物。 |
| 4. | According to the primers sequences , which were published by kelly , a 1071 - bp fragment containing the enzyme coding region of fut2 gene was amplified by pcr
参考kelly等发表的序列设计引物，扩增fut2基因蛋白编码区片段( 1071bp ) ，进行rflp分析。 |
| 5. | Using the tpsl gene digested form the prokaryotic expressing vector as template , according to the published sequence , pcr primers of tpsl gene was designed , 1500bp fragment of tpsl was generated
以从t - vector中酶切得到的tps1为模板，根据已知序列设计pcr引物，扩增海藻糖- 6 -磷酸合成酶基因( tps1 ) 。 |
| 6. | Two pairs of primers were designed based on the sequence of mddl to get a 0 . 9kb and an 1 . 1kb pcr products which were confirmed to be expected sequence from mc2155 by dna sequencing
根据该基因的序列设计了两对特异引物，以mc ~ 2155dna为模板，扩增得到0 . 9kb和1 . 1kb的产物。其中的0 . 9kb产物经测序验证与mddl的序列一致。 |
| 7. | Two oligonucleotides were synthesized according to the sequence of p . furiosus extracellular a - amylase gene amya through the genbank and used as primers for pcr with p . juriosus genomic dna as the template
根据genbank公布的p furiosus的-淀粉酶基因amya序列设计两条引物，以p furiosus的基因组dna为模板进行pcr 。 |
| 8. | Two special primers of race were designed based on the above sequence of the rt - pcr product . two fragments of 5 ' cdna ( 895bp ) and 3 ' cdna ( 581 bp ) were amplified by race reaction
以此特异片段设计特异引物，采用race技术分别获得-葡萄糖苷酶cdna3 ’端581bp和5 ’端895bp片段，后再依据上述两段序列设计一对引物，扩增出酶的全长序列1475bp 。 |
| 9. | Which shared 10 % ~ 73 % identities to other rips from plants and 37 % ~ 73 % to other rjps from cucurbitaceae . six novel rip gene fragments ( 408 ' bp ) , 1 from benincasa hispida and 5 from cucurbit a moschaia
根据葫芦科rip上、下游两段高度保守的氨基酸序列设计简并引物对ly1 ly2 ，对基因组dna进行pcr扩增，首次建立了rip新基因快速筛选体系。 |
| 10. | In this study , an escherichia coli strain with high phytase activity was screened from pig excreta . using primers designed according to the sequence originally described by dassa , appa gene was amplified by pcr technique
本实验从猪粪中筛选出一个高产植酸酶的e . coli菌株，根据dassa等报道的序列设计一对引物通过pcr方法扩增了appa基因。 |
