English translation for "小鼠胚胎成纤维细胞"
|
- mouse embryo fibroblasts
mouse embryonal fibroblast cell
mouse embryonic fibroblasts
pmef
Related Translations:
胚胎病: embryonizationembryopathiaembryopathy 胚胎营养: embryometrophiaembryometrophyembryometrotrophiaembryotrophy 胚胎遗迹: embryomorphousvestigevestigium 胚胎性癌: carcinoma embryonalcarcinoma embryonicembryonal carcinomaembryonalcarcinomaembryonic carcinoma
- Example Sentences:
| 1. | Isolation and culture of murine embryonic fibroblasts of kunming species in vitro
昆明小鼠胚胎成纤维细胞的分离与体外培养 | | 2. | We also investigated the factors including feeder layer cells , growth factors and complements , which may affect the cultivation and differentiation of these cells in vitro . the main contents as follows : ( 1 ) isolation and culture of mouse embryonic fibroblast ( pmef ) and preparation the feeder layers
主要内容包括： ( 1 )小鼠胚胎成纤维细胞( mef )的分离培养和饲养层细胞的制作以妊娠13 . 5天或14 16天的小鼠为材料，建立了mef分离培养体系。 | | 3. | Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml , es - d3 cells being used in our experiments formed normal clones , expressed akp and kept their normal karyotype over many passages . the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers
结果显示： eso3细胞在小鼠胚胎成纤维细胞上和或含白血病抑制因于亿f ）的es细胞培养液中形成典型的胚胎干细胞克隆，碱性磷酸酶染色结果为强阳性，具有正常二倍体核型以及具有在体内外分化为三个胚层来源的组织细胞的潜能，而且具有形成种系嵌合动物的能力。 | | 4. | Phopholipase c - 1 ( plc - 1 ) is widely known to play an important role in regulating cell proliferation and differentiation , development of the organisms , cell transformation and oxidative stress . till now , the mechanism how phopholipase c - 1 acts can not be thoroughly illustrated , nor has the interaction between plc - 1 pathway and other signal pathways been systematically reported . this research chose 2 - de + ms as the basic method from all kinds of proteomics strategies and compared the total protein expression map of mef genetically deficient in plc - 1 ( plc - 1 - / - ) to that of wild type mef ( plc - l + / + ) aimed to find some protein spots differentially expressed , thus we can discuss the impact of knockout of plc - 1 on signal transduction initiated by growth factors such as egf comprehensively . in this way , we can study the biological function of plc - 1 and mechanism of plc - 1 pathway indirectly , which will contribute a lot to further analysis
鉴于plc - 1发挥上述作用的机制尚未完全阐明， plc - 1通路与其他信号通路间的交联和代偿尚无系统报道，又因为以往的研究方法不够全面，本研究以野生型小鼠胚胎成纤维细胞( plc - 1 ~ ( + / + ) )和缺失磷脂酶c - 1的小鼠胚胎成纤维细胞( plc - 1 ~ ( - / - ) )为研究模型，在众多蛋白组学策略中选择了双向电泳+质谱( 2 - de + ms )作为研究手段，通过对比表皮生长因子( egf )刺激24小时后上述两种细胞的总蛋白质表达差异，全面地探讨敲除plc - 1对生长因子诱导的信号传递的影响，从而间接研究plc - 1生物学作用、信号传递机制及其代偿情况，为后续的深入研究打下基础。 | | 5. | The primary primordial germ cells is obtain from the human embryos after 4 - 8 weeks of pregnancy . after mechanical desection and enzymic digestion , the cells were cultured on mouse embryonic flbroblast or human embryonic flbroblast feeder layer inactivated by mitomycin . the medium contains several cytokines : lif ( leukemia inhibitory factor ) , bfgf and foskolin
从4 ? 8周的流产胎儿的原始生殖嵴部位分离到原始生殖细胞，经过机械和化学方法的解离后培养于事先用丝裂霉素处理过的小鼠胚胎成纤维细胞或人胚胎成纤维细胞饲养层上，培养基中添加了lif ， bfgef ， foskolin等细胞因子，此后大约每7天左右传代一次。 |
- Similar Words：
- "小鼠脑脊髓炎病毒检测方法" English translation, "小鼠脑抗原" English translation, "小鼠脑钠素" English translation, "小鼠脑内半数致死量" English translation, "小鼠逆转录酶" English translation, "小鼠胚胎同种移植技术" English translation, "小鼠皮肤细胞的存活曲线" English translation, "小鼠皮肤肿瘤细胞的存活曲线" English translation, "小鼠皮质醇" English translation, "小鼠皮质酮" English translation
|
|
|
