| 1. | It is the appropriate time to add the organic algaecides at the prophase of logarithmic phase 有机除藻剂对棕囊藻赤潮最佳投药期是其对数生长期的前期。 |
| 2. | We also compared the effects of different inducible time , inducible temperature and iptg concentration . recombinant protein was inclusion body that was expressed at 37 C温度时,绘制pgex一4t一1一nk4一bl21的生长曲线,发现该凶在l小日、 {一场分时进入对数生长期。 |
| 3. | In nitrogen - free medium , the logarithmic phase of the strain w12 was about 13 - 48 h . in logarithmic period its doubling time was 2 - 4 h in nitrogen - free medium , 0 . 9 - lh when enough nk 在无氮培养基上该菌在13h后进入对数生长期,并持续至48 52h ,这期间其生长代时为2 - 4h ,有氮培养基上为0 . 9 - 1h 。 |
| 4. | The protein expression profiles of rt19 growing in exponential phase after being treated with no nacl and lmol / l nacl for 5 min and 50 min were analyzed by differentially displayed proteome 采用差异显示蛋白质组学方法,对对数生长期的rt19 (培养20小时)在无盐和盐激5min 、 50min条件下的蛋白表达谱进行研究。 |
| 5. | 2 . the plasimd encoding human carcinoembryonic antigen was transfected with lipofectine into human dcs and human tumor cells such as mgc803 , ls174t and eel09 . the culture supernatants were collected for assaying carcinoembryonic antigen by . 3 1640培养液中,在37ac , 5 co ,的培养箱内培养, ’每3心传代一次,取对数生长期的细胞进行实验。 |
| 6. | The beginning of exponential growth phase for jsa10 was 8th day , with a rapid growth phase from 8th to 12th day followed by a slower growth phase from 14th to 18th day , then entered a senescence phase after 20th day Jsa25与jsa10相比,生长周期较短,进入对数生长期提前2d ,增长高峰期短,较快进入生长缓慢期,花色素苷的积累高峰也是在生长缓慢期。 |
| 7. | The presence of calcium is beneficial to obtain higher tryptophanase activity . the activity of tryptophanse expressed by addition of appropriate concerntration of iptg is 91 - fold higher than that of no induction . the highest specific activity was 0 . 2mmiptg对菌体的生长有抑制作用,在细胞对数生长期前期诱导抑制最强烈, 0 . 1mmiptg基本不抑制生长;临界浓度0 . 15mm的iptg和37ac是比较好的组合。 |
| 8. | Jsa10 ( magenta colored line ) and jsa25 ( orange colored line ) were selected from subculture . both of them showed similar " s - type " growth curve , of which jsa10 was better in callus growth and pigmentation , and the period of anthocyanin production was longer Jsa10和jsa25的愈伤组织生长曲线均呈“ s ”形, jsa10在第8d进入对数生长期, 8d 12d是愈伤组织的快速生长期, 14d 18d是愈伤组织生长缓慢期, 20d以后是愈伤组织衰老期。 |
| 9. | Was supplied . its essential characteristics are as follows : short rods , 2 . 0x1 . 6 ^ m in diameter , arranged singly , gram negative , facultatively anaerobic , having both a respiratory and a fermentative type of metabolism , oxidase negative and catalase positive 通过形态观察及生理生化特征测定发现菌株w12为革兰氏阴性菌,短杆状,对数生长期细胞大小为: 2 . 0 1 . 6 m ,单个排列;兼性厌氧,氧化酶阴性,发酵葡萄糖产酸产气,不运动。 |
| 10. | 2 - e4 and s2 is induced respectively by 8 - ag and 5 - brdu with different drug concentration to make them deficient in hypoxanthine - guanine phosphoribosyl transferase ( hgprt ) and in thymidine kinase ( tk ) respectively and renamed 2 - e4 - a and 82 - 6 . their antibodies " isotypes are tested by goad anti - mouse isotype regent 取驯化好并处于对数生长期的2 - e _ 4 - a和s _ 2 - b细胞,再常规融合和筛选,三次克隆化后得稳定分泌双特异性抗体的杂交-杂交瘤细胞株6株。 |