English translation for "大片段"

kluyveromyces

Related Translations:

大片:  blockbusterhunk

大片森林:  large stretches of forests

大片目标:  volume target

大片污渍:  blotch

大片土地:  tract

大片刨花:  wafer

大片陆地:  landmass

大片水肿:  massive edema

大片工艺:  full slicefull-slice

大片浮冰:  floe

Example Sentences:

	1.	Other times these smaller pieces join together into larger pieces , including pieces that are larger than the original molecule 另一些时候这些小片段会合大片段融合，形成比原来更大的分子。
	2.	Plasmid prd29a - gus was double - digested by smal + ecl136 , the larger fragment was collected and purified , and then self - ligated of blunt end , middle vector pvct2011 with rd29a - tnos fragment was constructed Prd29a - gus经smai + ecl136双酶切后，回收大片段，平端线型dna自环化，构建成携带rd29a一tnos基因片段的中间载体pvct20ll 。
	3.	Many streptomyces species exhibit a very high degree of genetic instability which is usually manifested as genomic rearrangement such as large deletion , and high - level dna amplification of those sequences flanking the deletion 许多链霉菌表现出高度的遗传不稳定性，通常为大片段缺失或基因组重排，以及缺失区域两侧序列的高水平dna扩增。
	4.	3 . the amplified dna fragment was then ligated into the hindlll and ecori sites of pmg36e . the ligation mixture was transformed into jm109 for the initial cloning . the recombinant plasmid pmg36e / nisa isolated from jm109 transformants were analyzed by restriction enzyme digestion 将酶切并纯化后的pcr扩增产物与大片段连接并转化e . colijm109 ，在含红霉素的lb平板上筛选到含有重组质粒的转化子。
	5.	The total efficiency of coinjection was 2 . 08 % . this study obtained the results and advances as follows : 1 . twelve transgenic mice were successfully produced by coinjection with three hum an dna fragments - hsa dna , hfenl and an uncoding cit987sk - 384d8 非编码大片段dnacit987sk 384d8对况a基因和hfeni基因整合有某种促进作用，这与内含子对基因整合有促进作用相吻合，也与brinster的研究结果一致。
	6.	Correct clones were selected and plasmid dna was isolated and digested with saci and puvii . a dna fragment of about 2 . 1kb was purified and labeled by dig - 11dutp as probe . at least 40 positive clones were obtained from human genomic library by in situ colony hybridyzation with this probe . among them one clone contains human serum album dna by sequence 以pcr扩增的人血清白蛋白( hsa )基因片段为探针，从人的基因组文库中杂交筛选的阳性克隆中，经测序分析，有一个克隆含有全长hsadna ，用从其它的阳性克隆中选取两种dna片段，即dna修复基因hfen1和一段非编码大片段cit987sk - 384d8 ，与人hsadna一起，进行显微共注射，成功制备了转多基因小鼠。
	7.	To facilitate the detection of gene replacement , apr gene was placed in the middle of the two inserts . orit from e . coli plasmid rk2 was also incoporated into the vector , therefore , pij903 derivatives can be mobilized from e . coli into streptomyces sp . fr - 008 by either bi - parental conjugation or by conjugation between streptomyces 为了在克隆完整基因簇时避免这两方面的影响并能随机地获得dna大片段，我们将第二代yac载体pjs97 - pjs98改造成为适合于在白林泉链霉菌基因组中dna大片段的基因置换与克隆链霉菌中分析被克隆大片段功能的基因置换载体。
	8.	In this paper , three human dna fragments were coinjected into mice in order to study the influence of uncoding large dna fragment on hsa or hfen1 gene ' s integration efficiency , to explore methods to produce high expression of human serum album animals , to find out how to establish multi - transgenic animals in a way of saving time and money , to provide experience and reference to others . it mainly contains the following aspects : firstly , according to the sequence of human serum album gene cdna in genebank , one primer was designed 本实验以小鼠为研究对象，采用两种基因? hsacdna 、 hfen1和一种非编码大片段dna进行共注射，旨在探索非编码大片段dna对hsa和hfen1基因整合的影响及建立提高hsa基因整合效率的转基因动物的方法，同时，探索多基因转基因动物建立的策略，以便寻求一种既节省时间，而又简单经济的转基因方法，为以后的研究提供一点的探索性经验和参考。
	9.	Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment , three methods were used to extract genomic dna of bacillus subtilis , i . e . low melting - point agarose embedding method , sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method . the genomic dna of bacillus subtilis were gained by these methods , and the operated programs of the methods were improved . the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods 大片段基因组dna的提取为了获得用于pcr扩增(长距离pcr扩增和分段pcr扩增)的高纯度、大片段(至少为pcr产物长度的4倍)的dna模板，应用三种方法：低熔点琼脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和细菌基因组dna提取试剂盒法，分别提取获得了枯草杆菌基因组dna ，并对3种方法的操作程序进行了不同程度的改进，结果表明：低熔点琼脂糖包埋法提取的基因组dna片段明显大于后两种方法，采用0 . 5琼脂糖凝胶电泳3h ，仍然跑不出加样孔。
	10.	In 2001 , wilson and his colleagues cloned another two members - human wnk1 gene and wnk4 gene which were located on chromosome 12 and 17 , respectively . the two genes are disease - causing genes responsible for a mendelian hypertension . the disease - causing mutations are the large deletions in the first intron in wnk1 gene , causing increased expression in leukocytes 2001年， wilson等克隆了此家族的另两个成员? ?人的wnk1和wnk4基因，分别位于12号和17号染色体，这两个基因是一种孟德尔遗传型高血压病的致病基因，致病突变分别是wnk1基因第一内含子的大片段缺失，导致患者白细胞的基因表达量提高5倍左右。

Similar Words：

"大偏转角扫描器" English translation, "大偏转角扫描器 宽角扫描器" English translation, "大片" English translation, "大片,片,板,单子,张,床单,被单" English translation, "大片冰层" English translation, "大片段而设计的置换型噬菌体载体系列" English translation, "大片肺萎病" English translation, "大片分层 表面分层 表皮分层" English translation, "大片浮冰" English translation, "大片工艺" English translation