| 1. | They clearly demonstrated that nitrogenase could catalyze the reduction of acetylene as well as n2
他们明确地证明，固氮酶能催化乙炔以及N2的还原。 |
| 2. | Dinitrogenase the enzyme that catalyzes nitrogen fixation
固氮酶：能催化固氮反应的酶。 |
| 3. | They clearly demonstrated that nitrogenase could catalyze the reduction of acetylene as well as n2
他们明确地证明，固氮酶能催化乙炔以及n2的还原。 |
| 4. | An auto - controlled fermentor was used to culture these strains under nitrogen fixation conditions and the nitrogneases were effectively depressed
在固氮条件下，所构建固氮酶突变菌株均表现为严格的nif ~ -表型，不能进行固氮生长。 |
| 5. | ( 4 ) h2 evolution by mofe protein with a - gln194 and citrate substitutions was inhibited by co at 32 % inhibition rate , comparing to 71 % of that for nifv - mofe protein
Co对nifv ~ - mofe蛋白放氢的最大抑制率为71 ， - gln ~ ( 194 )和柠檬酸双重替换使的co抑制固氮酶放氢的最大抑制率下降到31 。 |
| 6. | Analysis of the sequence revealed that adda resembled nifs of nitrogen - fixing bacteria and dnda . the entire add gene cluster showed 78 % identity to dnd gene cluster from s . lividans
同源性比较揭示add基因簇的adda基因与固氮酶基因nifs高度同源， add与变铅青链霉菌dnd核苷酸的同一性为78 。 |
| 7. | 2 . proceedings of the reseaches on the novel nitrogenase mnfe protein and crfe protein several new batches of mnfe protein and crfe protein were prepared . their substrate reduction activities have been determined by the complementation with uw45 fe protein
新型固氮酶mnfe蛋白和crfe蛋白的特性与结晶研究在已有的工作基础上，分离纯化了几批mnfe蛋白和crfe蛋白，并用部分纯的ugh fe蛋白进行活性互补，分别测定了mnfe蛋白和crfe蛋白的底物还原活性。 |
| 8. | The paper reviews home and overseas research works on exhumation and use of nitrogen - fixing resource , genetics engineering of nitrogen fixation , and biochemistry of nitrogenase and chemical modeling of biological nitrogen fixation , and describe the prospect of biological nitrogen - fixing research
本文从固氮资源的发掘和利用、固氮的遗传工程以及固氮酶生物化学和化学模拟生物固氮三方面对国内外的研究进展进行了全面的阐述，并对生物固氮研究的前景进行了展望。 |
| 9. | Effects of diverse environmental factors on the growth rate ( od4oo ) and nitrogenase activity ( ara ) of the strain w12 hi nitrogen - free culture were investigated in our experiments . the results implied that the strain w12 could easily adapt to different cultural conditions : it could use various carbon sources ( especially glucose , sucrose , malic acid , mannitol ) , propagate quickly and fix nitrogen at a temperature range of 15 ? to 40 ? and at 25 - 35 ? for optimum , at a ph range of 4 to 8 . 5 , at a saline concentration range of 0 . 01 % to 1 . 5 % ; low nlv " concentration had little effect on its nitrogenase activity . ara could also be detected when it grow in the culture media with 5mmol / l ntv "
W12菌株对环境因子的适应性研究：无氮培养条件下，测定温度、碳源、酸碱度、渗透压对w12生长及固氮能力的影响，结果表明，在15 - 40下均能生长并表达固氮酶活性，其最适生长及固氮的温度为25 - 35 ；能利用葡萄糖、蔗糖、苹果酸、甘露醇等多种碳源生长并固氮，当培养基中同时存在蔗糖和苹果酸时，细菌生长和固氮活性最强；在偏酸和偏碱的条件下( ph4 . 5 - 8 . 5 )均能保持较强的生长势和较高的固氮酶活性，并能通过调节自身代谢平衡并适应环境的酸、碱性变化，使培养液趋于中性：能耐受较高的渗透压，培养液中卜、 5 naci浓度对其生长和固氮酶活性影响不大，当naci浓度升至2时，菌株的生长势及固氮酶活性才有所下降：低浓度的铰对其固氮酶活性影响不大，在0 |
| 10. | Comparison of substrate - reduction properties of these altered mofe proteins with wt and nifv - mofe proteins showed that : ( 1 ) only the a - gln194 substitution did not affect the total electron to proton reduction and notably double altered mofe protein with substitution of citrate and a - lys190 only retained very poor proton reduction activity . ( 2 ) a - gln194 substitution made a more direct effect on n2 reduction then other two substitutions
四个突变体固氮酶、野生型和nifv ~ -固氮酶的mofe蛋白的底物还原特性比较表明： ( 1 ) - gln ~ ( 194 )替换不影响固氮酶还原质子时的总电子流；尤其引人注意的是，含有- lys ~ ( 190 )和柠檬酸双重替换的mofe蛋白几乎完全失去了质子还原的能力。 |
